해양퇴적토에 오염된 다환방향족탄화수소 분해 증진을 위한 bioreactor 조건 연구

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are a kind of toxic environmental pollutant, accumulated usually in marine sediments. Due to the potential hazardous to human, removal of PAHs from sediments has been great concern. In the present sturdy, the effect of microbial inoculation and the supplementation of mixed form cyclodextrin (M-CD) was assessed in the pre-sterilized or unsterilized microcosms for optimizing operational conditions of bioslurry reactor for ex situ bioremediation of sediment contaminated by PAHs. The activity og electron transport system was increased by the addition of M-CD. The degradation rate of PAHs in sterilized microcosms as app. 9-20% by the inoculation of single strain and 24-37% by the inoculation of microbial consortium supplemented with 1% M-CD, respectively. The degradation was not observed in microcosms without sterilization under the same conditions. Signals of inoculated bacteria were decreased to detection limit after 2 days in the microcosms without M-CD. As a conclusion, microbial inoculation with appropriate carbon sources and removal of natural flora and grazers are required for the efficient remediation of sediments contaminated by PAHs in ex situ bioreactor.