Molecular characteristics and biological activities of anionic macromolecules from Codium fragile SCIE SCOPUS

Cited 48 time in WEB OF SCIENCE Cited 55 time in Scopus
Title
Molecular characteristics and biological activities of anionic macromolecules from Codium fragile
Author(s)
Tabarsa, Mehdi; Karnjanapratum, Supatra; Cho, MyoungLae; Kim, Jin-Kyung; You, SangGuan
Publication Year
2013-08
Abstract
Water-soluble anionic macromolecules isolated from Codium fragile and fractionated using ion-exchange chromatography were investigated to determine their molecular characteristics and immunostimulating activity. The crude molecules and fractions (F-1, F-2, and F-3) consisted mostly of carbohydrates (44.1-80.5%), sulfates (3.2-22.2%) and proteins (3.0-15.7%) with small amounts of uronic acids (1.1-4.2%), and included different levels of mannose (91.3-18.7%), glucose (62.7-8.6%) and galactose (37.5-59.5%). These molecules contained one or two subfractions with molecular weights (M-w) ranging from 148 x 10(3) to 4879 x 10(3) g/mol. The crude, F-1 and F-2 stimulated RAW264.7 cells to produce considerable amounts of pro-inflammatory mediator nitric oxide (NO) and cytokines. The treatment of sample molecules facilitated the degradation of I kappa-B and phosphorylation of MAPK in RAW264.7 cells, suggesting that they might stimulate RAW264.7 cells through the activation of NF-kappa B and MAPK pathway. Proteins in fraction F-2 were essential to possess its bioactivity and its main backbone was composed of mixed linkages of (1 -> 3)-alpha and beta-D-mannan. (C) 2013 Elsevier B.V. All rights reserved.
ISSN
0141-8130
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3145
DOI
10.1016/j.ijbiomac.2013.04.022
Bibliographic Citation
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.59, pp.1 - 12, 2013
Publisher
ELSEVIER SCIENCE BV
Subject
SULFATED POLYSACCHARIDES; ENTEROMORPHA-INTESTINALIS; HYPSIZIGUS-MARMOREUS; ANTIVIRAL ACTIVITY; ANTITUMOR-ACTIVITY; ULVA-PERTUSA; GREEN-ALGA; IN-VITRO; GALACTAN; CHLOROPHYTA
Keywords
Codium fragile; Anionic macromolecules; Immunomodulation; Glycosidic linkage; NF-kappa B; MAPK pathway
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE BV
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