Molecular characteristics and biological activities of anionic macromolecules from Codium fragile
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Title
- Molecular characteristics and biological activities of anionic macromolecules from Codium fragile
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Author(s)
- Tabarsa, Mehdi; Karnjanapratum, Supatra; Cho, MyoungLae; Kim, Jin-Kyung; You, SangGuan
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Alternative Author(s)
- 조명래
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Publication Year
- 2013-08
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Abstract
- Water-soluble anionic macromolecules isolated from Codium fragile and fractionated using ion-exchange chromatography were investigated to determine their molecular characteristics and immunostimulating activity. The crude molecules and fractions (F-1, F-2, and F-3) consisted mostly of carbohydrates (44.1-80.5%), sulfates (3.2-22.2%) and proteins (3.0-15.7%) with small amounts of uronic acids (1.1-4.2%), and included different levels of mannose (91.3-18.7%), glucose (62.7-8.6%) and galactose (37.5-59.5%). These molecules contained one or two subfractions with molecular weights (M-w) ranging from 148 x 10(3) to 4879 x 10(3) g/mol. The crude, F-1 and F-2 stimulated RAW264.7 cells to produce considerable amounts of pro-inflammatory mediator nitric oxide (NO) and cytokines. The treatment of sample molecules facilitated the degradation of I kappa-B and phosphorylation of MAPK in RAW264.7 cells, suggesting that they might stimulate RAW264.7 cells through the activation of NF-kappa B and MAPK pathway. Proteins in fraction F-2 were essential to possess its bioactivity and its main backbone was composed of mixed linkages of (1 -> 3)-alpha and beta-D-mannan. (C) 2013 Elsevier B.V. All rights reserved.
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ISSN
- 0141-8130
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3145
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DOI
- 10.1016/j.ijbiomac.2013.04.022
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Bibliographic Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.59, pp.1 - 12, 2013
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Publisher
- ELSEVIER SCIENCE BV
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Subject
- SULFATED POLYSACCHARIDES; ENTEROMORPHA-INTESTINALIS; HYPSIZIGUS-MARMOREUS; ANTIVIRAL ACTIVITY; ANTITUMOR-ACTIVITY; ULVA-PERTUSA; GREEN-ALGA; IN-VITRO; GALACTAN; CHLOROPHYTA
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Keywords
- Codium fragile; Anionic macromolecules; Immunomodulation; Glycosidic linkage; NF-kappa B; MAPK pathway
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Type
- Article
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Language
- English
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Document Type
- Article
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