해양성 스트렙토마이세스에서 분리된 신규 항암물질
-
Title
- 해양성 스트렙토마이세스에서 분리된 신규 항암물질
-
Alternative Title
- A New Cytotoxic Compound from a Marine-derived Streptomyces sp.
-
Author(s)
- 정성윤; 김태식; 이희승; 신희재
- KIOST Author(s)
- Lee, Hyi Seung(이희승); Shin, Hee Jae(신희재)
-
Alternative Author(s)
- 정성윤; 김태식; 이희승; 신희재
-
Publication Year
- 2005-07-01
-
Abstract
- In the course of our screening program of bioactive marine natural products, we isolated a marine Actinomycete Streptomyces sp. from deep-sea sediments. This strain exhibited potent cytotoxic activity against human leukemia cell line(K-562). This strain was grown in modified Bennett\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\'s medium at 32C with shaking in a 5L glass flask containing modified Bennett\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\'s medium. After 7 days, the culture broth was centrifuged and then fltrated to obtain a cell-free supernatent, followed by extraction with EtOAC. The aqueous EtOAc layer was concentrated and the residual aqueous suspension(450mg) was subject to silica vacuum flash chromatography using gradient mixtures of n-hexan and EtOAc as eluents. The fractions eluted with 40%,k and 60% EtOAc in hexane from silica vacuum flash chromatography were combined(100 mg) and purified by reversed-phase HPLC to yield a pure compound (11.5 mg) as a colorless amorphous powder. Based upon the results of combined spectral analyses, the structure of this compound was determined to be a new compound. This compound exhibited significant cytotoxic activity against human leukemia cell line (K-562).
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/31402
-
Bibliographic Citation
- 2005 Annual Meeting and International Symposium on Microorganisms and Human Well-Being, pp.445, 2005
-
Publisher
- 한국미생물생명공학회
-
Type
- Conference
-
Language
- English
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.