해양성 스트렙토마이세스에서 분리된 신규 항암물질

Title
해양성 스트렙토마이세스에서 분리된 신규 항암물질
Alternative Title
A New Cytotoxic Compound from a Marine-derived Streptomyces sp.
Author(s)
정성윤; 김태식; 이희승; 신희재
KIOST Author(s)
Lee, Hyi Seung(이희승)Shin, Hee Jae(신희재)
Alternative Author(s)
정성윤; 김태식; 이희승; 신희재
Publication Year
2005-07-01
Abstract
In the course of our screening program of bioactive marine natural products, we isolated a marine Actinomycete Streptomyces sp. from deep-sea sediments. This strain exhibited potent cytotoxic activity against human leukemia cell line(K-562). This strain was grown in modified Bennett\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\'s medium at 32C with shaking in a 5L glass flask containing modified Bennett\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\'s medium. After 7 days, the culture broth was centrifuged and then fltrated to obtain a cell-free supernatent, followed by extraction with EtOAC. The aqueous EtOAc layer was concentrated and the residual aqueous suspension(450mg) was subject to silica vacuum flash chromatography using gradient mixtures of n-hexan and EtOAc as eluents. The fractions eluted with 40%,k and 60% EtOAc in hexane from silica vacuum flash chromatography were combined(100 mg) and purified by reversed-phase HPLC to yield a pure compound (11.5 mg) as a colorless amorphous powder. Based upon the results of combined spectral analyses, the structure of this compound was determined to be a new compound. This compound exhibited significant cytotoxic activity against human leukemia cell line (K-562).
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/31402
Bibliographic Citation
2005 Annual Meeting and International Symposium on Microorganisms and Human Well-Being, pp.445, 2005
Publisher
한국미생물생명공학회
Type
Conference
Language
English
Publisher
한국미생물생명공학회
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