초고온성 아케아 써모코커스 NA1의 녹말분해효소의 과발현 및 특성

DC Field Value Language
dc.contributor.author 임재규 -
dc.contributor.author 이현숙 -
dc.contributor.author 김윤재 -
dc.contributor.author 전정호 -
dc.contributor.author 이규호 -
dc.contributor.author 강성균 -
dc.contributor.author 이정현 -
dc.date.accessioned 2020-07-17T07:51:47Z -
dc.date.available 2020-07-17T07:51:47Z -
dc.date.created 2020-02-11 -
dc.date.issued 2005-10-13 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/31297 -
dc.description.abstract Genomic analysis of a hyperthermophilic archaeon Thermococcus NA1. revealed the presence of an open reading frame consisting of 1,377 bp similar to GH13 a-amylases from the genus Thermococcus and Pyrococcus. This amylolytic enzyme, designated TNA1_amyl (Thermococcus sp. NA1 amylase), was cloned and expressed in Escherichia coli. The full-length protein was detected in the culture supernatant, indicating that the signal peptide was processed in E. coli and thus the mature form of the protein was reconstructed based on N-terminal sequencing data of the secreted recombinant protein. The purified protein by His-tagged affinity chromatography was highly thermostable with an optimum temperature of 80°C, and an optimum pH of 5.5. More than 50% of the maximum activity was retained in the range between pH 5.0 and pH 9.5. The enzyme displayed thermostability with half-life (t1/2) of 10 min at 90°C, and the thermostability of TNA1_amyl was significantly enhanced in the presence of calcium with half-life (t1/2) of 153 min at 90°C. TNA1_amyl hydrolyzed maltooligosaccharides, amylopectin and starch, not pullulan to produce mainly G2 to G7. -
dc.description.uri 2 -
dc.language English -
dc.publisher 한국미생물연합회 -
dc.relation.isPartOf 한국미생물연합회 프로시딩 -
dc.title 초고온성 아케아 써모코커스 NA1의 녹말분해효소의 과발현 및 특성 -
dc.title.alternative Overexpression and characterization of an alpha amylase from the hyperthermophilic archaeon THermococcus sp. NA1 -
dc.type Conference -
dc.citation.conferencePlace KO -
dc.citation.endPage 263 -
dc.citation.startPage 263 -
dc.citation.title 한국미생물연합회 프로시딩 -
dc.contributor.alternativeName 임재규 -
dc.contributor.alternativeName 이현숙 -
dc.contributor.alternativeName 김윤재 -
dc.contributor.alternativeName 전정호 -
dc.contributor.alternativeName 강성균 -
dc.contributor.alternativeName 이정현 -
dc.identifier.bibliographicCitation 한국미생물연합회 프로시딩, pp.263 -
dc.description.journalClass 2 -
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