초고온성 아케아 써모코커스 NA1의 녹말분해효소의 과발현 및 특성
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 임재규 | - |
dc.contributor.author | 이현숙 | - |
dc.contributor.author | 김윤재 | - |
dc.contributor.author | 전정호 | - |
dc.contributor.author | 이규호 | - |
dc.contributor.author | 강성균 | - |
dc.contributor.author | 이정현 | - |
dc.date.accessioned | 2020-07-17T07:51:47Z | - |
dc.date.available | 2020-07-17T07:51:47Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2005-10-13 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/31297 | - |
dc.description.abstract | Genomic analysis of a hyperthermophilic archaeon Thermococcus NA1. revealed the presence of an open reading frame consisting of 1,377 bp similar to GH13 a-amylases from the genus Thermococcus and Pyrococcus. This amylolytic enzyme, designated TNA1_amyl (Thermococcus sp. NA1 amylase), was cloned and expressed in Escherichia coli. The full-length protein was detected in the culture supernatant, indicating that the signal peptide was processed in E. coli and thus the mature form of the protein was reconstructed based on N-terminal sequencing data of the secreted recombinant protein. The purified protein by His-tagged affinity chromatography was highly thermostable with an optimum temperature of 80°C, and an optimum pH of 5.5. More than 50% of the maximum activity was retained in the range between pH 5.0 and pH 9.5. The enzyme displayed thermostability with half-life (t1/2) of 10 min at 90°C, and the thermostability of TNA1_amyl was significantly enhanced in the presence of calcium with half-life (t1/2) of 153 min at 90°C. TNA1_amyl hydrolyzed maltooligosaccharides, amylopectin and starch, not pullulan to produce mainly G2 to G7. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국미생물연합회 | - |
dc.relation.isPartOf | 한국미생물연합회 프로시딩 | - |
dc.title | 초고온성 아케아 써모코커스 NA1의 녹말분해효소의 과발현 및 특성 | - |
dc.title.alternative | Overexpression and characterization of an alpha amylase from the hyperthermophilic archaeon THermococcus sp. NA1 | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 263 | - |
dc.citation.startPage | 263 | - |
dc.citation.title | 한국미생물연합회 프로시딩 | - |
dc.contributor.alternativeName | 임재규 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 김윤재 | - |
dc.contributor.alternativeName | 전정호 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | 한국미생물연합회 프로시딩, pp.263 | - |
dc.description.journalClass | 2 | - |