마이크로어래이법을 이용한 TBT 반응 발현 유전자의 탐색

Abstract

Tributyltin (TBT) is the most widely used as biocides in marine, wood, industrial cooling system and paper mills. Several techniques allow either the measurement of the TBT or its effect on living organisms. We determined TBT-inducible genes using a high-density microarray containing a nearly complete set of 4,289 annotated open reading frames in Escherichia coli. To determine temporal changes in gene expression across time courses after TBT treatment, we prepared a common reference RNA pool. We compared transcriptome levels of untreated and TBT treated cultures to common reference. Statistical analysis showed that approximately 200 genes are significantly induced by TBT treatment (400 ppb, w/v). Sixty-two of those genes are highly induced more than 4.0-fold by TBT treatment. The majortity of TBT-inducible genes are highlighted at 100 min after TBT treatment. The most highly induced pflC gene (probable pyruvate formate lyase activating enzyme 2) showed the induction ratio over 35.0-fold. Interestingly, fourteen ribosomal protein genes are singificantly induced over 4.0-fold by TBT. The results suggest that TBT may be involved in stimulating ribosomal protein biosynthesis. Our data propose that these TBT-inducible genes can be useful for assessment of the biological effects of TBT on living organism.