Expression of three novel cytochrome P450 (CYP) and antioxidative genes from the polychaete, Perinereis nuntia exposed to water accommodated fraction (WAF) of Iranian crude oil and Benzo[alpha]pyrene
SCIE
SCOPUS
Cited 35 time in
WEB OF SCIENCE
Cited 37 time in
Scopus
-
Title
- Expression of three novel cytochrome P450 (CYP) and antioxidative genes from the polychaete, Perinereis nuntia exposed to water accommodated fraction (WAF) of Iranian crude oil and Benzo[alpha]pyrene
-
Author(s)
- Won, Eun-Ji; Rhee, Jae-Sung; Shin, Kyung-Hoon; Jung, Jee-Hyun; Shim, Won Joon; Lee, Young-Mi; Lee, Jae-Seong
- KIOST Author(s)
- Jung, Jee Hyun(정지현); Shim, Won Joon(심원준)
-
Alternative Author(s)
- 정지현; 심원준
-
Publication Year
- 2013-09
-
Abstract
- To report a novel CYP genes and to evaluate its potency as a biomarker for oil pollution, we cloned three CYP genes and measured their expression profiles under controlled lab conditions using real-time reverse transcription PCR (real-time RT-PCR) after exposure of the water accommodated fraction (WAF) of Iranian crude oil and benzo[alpha]pyrene (B[alpha]P) as a positive control. Of these, CYP432A1 (CYP3 clan) gene was significantly induced by B[alpha]P exposure, indicating that the CYP3 clan gene would play an important role in polycyclic aromatic hydrocarbon (PAH) metabolisms, particularly for B[alpha]P in this species. However, the Perinereis nuntia CYP431A1 mRNA, a CYP2 clan gene, was sensitively expressed to WAF exposure with other two CYP genes. As one of Phase II detoxification enzymes, the glutathione S-transferase (GST) genes also upregulated with other antioxidant genes (SOD and CAT), indicating that WAF-exposed P. nuntia was properly responding to this kind of chemical stress. Thus, three CYP genes from the polychaete, P. nuntia have a potential as a biomarker in monitoring of the marine sediment after an oil spill accident. (C) 2013 Elsevier Ltd. All rights reserved.
-
ISSN
- 0141-1136
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3125
-
DOI
- 10.1016/j.marenvres.2013.05.014
-
Bibliographic Citation
- MARINE ENVIRONMENTAL RESEARCH, v.90, pp.75 - 84, 2013
-
Publisher
- ELSEVIER SCI LTD
-
Subject
- POLYCYCLIC AROMATIC-HYDROCARBONS; RAINBOW-TROUT; NEREIS-VIRENS; TOXICITY; BIOACCUMULATION; CADMIUM; SPILL; IDENTIFICATION; METABOLISM; SEA
-
Keywords
- Polychaete; Perinereis nuntia; Cytochrome P450; Water accommodated fraction WAF; Benzo[alpha]pyrene; Antioxidant genes
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.