호열성 dUTPase의 특성과 PCR 응용
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조요나 | - |
dc.contributor.author | 이현숙 | - |
dc.contributor.author | 김윤재 | - |
dc.contributor.author | 배승섭 | - |
dc.contributor.author | 임재규 | - |
dc.contributor.author | 강성균 | - |
dc.contributor.author | 이정현 | - |
dc.date.accessioned | 2020-07-17T06:52:40Z | - |
dc.date.available | 2020-07-17T06:52:40Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2006-05-03 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/31111 | - |
dc.description.abstract | Genomic analysis of a hyperthermophilic archaeon Thermococcus sp. NA1(TNA1) revealed the presence of an open reading frame consisting of 468 bp similar to dUTPases from Pyrococcus woesei and P. furiosus (83% identity and 93% similarity). TNA1 dUTPase has five motifs conserved in all dUTPases from eukarya and prokarya origins. The dUTPase gene was cloned and expressed in Escherichia coli. The purified protein behaved as a dimer in gel filtration and was able to hydrolyse both dUTP and dCTP. The optimum activity toward dUTP occurred at 80°C and pH 8.0. Moreover, the enzyme activity was highly dependent on Mg2+ concentration with optimum activity at 1mM to 5mM which is usually used at PCR amplification. TNA1 dUTPase activity was a little affected by KCl and (NH4)2SO4, while Tritoxn X-100 slightly increased dUTPase activity. The enzyme displayed thermostability with half-life (t1/2) of 170 min at 95°C. TNA1 dUTPase enhanced the PCR in a broader range of target lengths in combination with TNA1 DNA polymerase. Moreover, TNA1 dUTPase increased the yield of PCR products as much as other archaeal DNA polymerases. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국미생물학회 | - |
dc.relation.isPartOf | 2006(봄)한국미생물학회 | - |
dc.title | 호열성 dUTPase의 특성과 PCR 응용 | - |
dc.title.alternative | Characterization of dUTPase from the Hyperthermophilic Archaeon Thermococcus sp. NA1 and its Application to PCR | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 197 | - |
dc.citation.startPage | 197 | - |
dc.citation.title | 2006(봄)한국미생물학회 | - |
dc.contributor.alternativeName | 조요나 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 김윤재 | - |
dc.contributor.alternativeName | 배승섭 | - |
dc.contributor.alternativeName | 임재규 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | 2006(봄)한국미생물학회, pp.197 | - |
dc.description.journalClass | 2 | - |