해양 메타게놈으로부터 신규의 지질분해효소 분리
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 전정호 | - |
dc.contributor.author | 임재규 | - |
dc.contributor.author | 김준태 | - |
dc.contributor.author | 이현숙 | - |
dc.contributor.author | 강성균 | - |
dc.contributor.author | 김상진 | - |
dc.contributor.author | 이정현 | - |
dc.date.accessioned | 2020-07-17T06:31:18Z | - |
dc.date.available | 2020-07-17T06:31:18Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2006-06-19 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/30963 | - |
dc.description.abstract | Metagenomic libraries of various marine sediments collected from a seamount near hydrothermal vent, Artic coast (Dasan station) and inter tidal flat of Kangwha island were constructed using a fosmid vector. The total size of the metagenomic library was estimated to about 8 Gb (more than 200,000 clones). The library was screened for the lipolytic activity by plating the clones on TBN plate, and as a result, eighteen positive clone forming a halo were obtained. The genes representing the lipolytic activities were cloned. The sequence analysis revealed that all of 18 genes contained an oxyanion hole (XG) and an active-site (GXSXG) consensus region, and showed a significant similarity to α/β hydrolase family, essentially esterase/lipases. In an attempt to expression the esterase/lipase genes in E. coli, most of those enzymes were not produced in a soluble form. Several approaches to resolve the solubility issue are under progress. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국미생물생명공학회 | - |
dc.relation.isPartOf | 2006년 한국미생물생명공학회 | - |
dc.title | 해양 메타게놈으로부터 신규의 지질분해효소 분리 | - |
dc.title.alternative | Novel lipolytic enzymes from marine metagenomes approach | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 340 | - |
dc.citation.startPage | 340 | - |
dc.citation.title | 2006년 한국미생물생명공학회 | - |
dc.contributor.alternativeName | 전정호 | - |
dc.contributor.alternativeName | 김준태 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | 2006년 한국미생물생명공학회, pp.340 | - |
dc.description.journalClass | 2 | - |