Vibrio sp. GMD509의 신규 esterase의 ofloxacin ester의 광학특이성 분해 특성분석

DC Field Value Language
dc.contributor.author 박상이 -
dc.contributor.author 김준태 -
dc.contributor.author 강성균 -
dc.contributor.author 최형태 -
dc.contributor.author 김상진 -
dc.date.accessioned 2020-07-17T06:31:11Z -
dc.date.available 2020-07-17T06:31:11Z -
dc.date.created 2020-02-11 -
dc.date.issued 2006-06-19 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/30961 -
dc.description.abstract Vibrio sp. GMD509, a lipolytic marine bacterium isolated from the egg of sea hare. The esterase-encoding gene was cloned by screening genomic library of Vibrio sp. GMD509 and the sequence was determined. Similarity analysis revealed that the esterase gene consisted of 1017 bp (338 aa) with an estimated molecular mass of 37 kDa. The consensus sequence, G-X1-S-X2-G found in most serine esterase/lipases was also conserved, and the comparison of the deduced amino acids showed a high similarity to the enzymes of dienelactone hydrolase family. The esterase was overexpressed in E. coli and purified to homogeneity. The purified enzyme (rVlip509) was applied to the chiral resolution process of ofloxacin ester to test the potential usage as a biocatalyst. As a result, rVlip509 could hydrolyzed preferentially (S)-ofloxacin ester with eep value of 42.9 %. The novel esterase in this study might be effectively used as a novel biocatalyst for production of levofloxacin in the pharmaceutical industry. -
dc.description.uri 2 -
dc.language English -
dc.publisher 한국미생물생명공학회 -
dc.relation.isPartOf 2006년 한국미생물생명공학회 -
dc.title Vibrio sp. GMD509의 신규 esterase의 ofloxacin ester의 광학특이성 분해 특성분석 -
dc.title.alternative Characterization of a Novel Esterase Displaying Enantioselective Hydrolyzing Activity toward (S) - ofloxacin ester from the Marine Bacterium Vibrio sp. GMD509 -
dc.type Conference -
dc.citation.conferencePlace KO -
dc.citation.endPage 329 -
dc.citation.startPage 329 -
dc.citation.title 2006년 한국미생물생명공학회 -
dc.contributor.alternativeName 박상이 -
dc.contributor.alternativeName 김준태 -
dc.contributor.alternativeName 강성균 -
dc.contributor.alternativeName 김상진 -
dc.identifier.bibliographicCitation 2006년 한국미생물생명공학회, pp.329 -
dc.description.journalClass 2 -
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