초고온성 고세균 써모코커스 NA1 균주로부터 새로운 고온성 DNA 중합효소
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김윤재 | - |
dc.contributor.author | 이현숙 | - |
dc.contributor.author | 조요나 | - |
dc.contributor.author | 강성균 | - |
dc.contributor.author | 김상진 | - |
dc.contributor.author | 권석태 | - |
dc.contributor.author | 이정현 | - |
dc.date.accessioned | 2020-07-17T06:31:01Z | - |
dc.date.available | 2020-07-17T06:31:01Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2006-06-24 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/30955 | - |
dc.description.abstract | Genomic analysis of Thermococcus sp. NA1 revealed the presence of a family B type DNA polymerase gene (TNA1_pol) consisting of 3,927 bp. Sequence analysis revealed that the gene contained a putative 3'-5' exonuclease domain, a DNA polymerase domain and one in-frame intervening sequence of 1,605 bp (535 amino acids; intein), which is located in the middle of PolIII region. The mature form of the DNA polymerase without intein (TNA1_pol) was overexpressed in Esherichia coli, purified by metal affinity chromatography, and further characterized. Optimal activity of TNA1_pol was observed at pH 7.5 and 75 C. TNA1_pol was highly thermostable with t1/2 of 3.5 h at 100 C and 12.5 h at 95 C, respectively. The optimum condition for PCR amplification was determined, and the PCR parameters (error-rate, processivity and extension rate) of TNA1_pol were measured in comparison with rTaq, Pfu and KOD polymerases. TNA1_pol introduced 1 bp error in 4.45 kb on average in PCR amplification. The processivity of TNA1_pol was determined as 150 nt, and the extension rate of TNA1_pol was measured as 60 bases/sec, comparable to those of rTaq DNA polymerase. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | GIM | - |
dc.relation.isPartOf | 10-th Internation Symposium om the Genetics of Industrial Microorganisms | - |
dc.title | 초고온성 고세균 써모코커스 NA1 균주로부터 새로운 고온성 DNA 중합효소 | - |
dc.title.alternative | A novel DNA Polymerase from a Hyperthermophilic Archaeon Thermococcus sp. NA1 | - |
dc.type | Conference | - |
dc.citation.endPage | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | 10-th Internation Symposium om the Genetics of Industrial Microorganisms | - |
dc.contributor.alternativeName | 김윤재 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 조요나 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | 10-th Internation Symposium om the Genetics of Industrial Microorganisms, pp.1 | - |
dc.description.journalClass | 1 | - |