Isolation and characterization of a Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis
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Cited 42 time in
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Title
- Isolation and characterization of a Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis
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Author(s)
- Han, Jee Eun; Kim, Ji Hyung; Hwang, Sun Young; Choresca, Casiano H., Jr.; Shin, Sang Phil; Jun, Jin Woo; Chai, Ji Young; Park, Yong Ho; Park, Se Chang
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Alternative Author(s)
- 김지형
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Publication Year
- 2013-10
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Abstract
- A lytic bacteriophage (phage), designated SAH-1, was isolated from sewage effluent near a dairy cow farm in Gwacheon, South Korea to search for biocontrol agents against Staphylococcus aureus infections. The SAH-1 was morphologically classified as Myoviridae and possessed an approximate 144 kb double-stranded genomic DNA. The phage showed broad host ranges within S. aureus strains including methicillin-resistant strains, and its latent period and burst size were approximately 20 min and 100 PFU/cell, respectively. Moreover, morphologic and genomic analysis of SAH-1 revealed that the phage was closely related to other Myoviridae phages infecting Staphylococcus species. The bacteriolytic activity of phage SAH-1 at a multiplicity of infection (MOI) 1 and 100 indicated its efficiency for reducing bacterial growth. Based on these results, phage SAH-1 could be considered a potential therapeutic or prophylactic candidate against S. aureus infections. (C) 2013 Elsevier Ltd. All rights reserved.
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ISSN
- 0034-5288
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3091
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DOI
- 10.1016/j.rvsc.2013.06.001
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Bibliographic Citation
- RESEARCH IN VETERINARY SCIENCE, v.95, no.2, pp.758 - 763, 2013
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Publisher
- ELSEVIER SCI LTD
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Subject
- BOVINE MASTITIS; MANAGEMENT; RESISTANCE; GENOME; GENE
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Keywords
- Bacteriophage; Mastitis; Myoviridae; SAH-1; Staphylococcus aureus
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Type
- Article
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Language
- English
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Document Type
- Article
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