Isolation and characterization of a Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis SCIE SCOPUS

Cited 22 time in WEB OF SCIENCE Cited 23 time in Scopus
Title
Isolation and characterization of a Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis
Author(s)
Han, Jee Eun; Kim, Ji Hyung; Hwang, Sun Young; Choresca, Casiano H., Jr.; Shin, Sang Phil; Jun, Jin Woo; Chai, Ji Young; Park, Yong Ho; Park, Se Chang
Publication Year
2013-10
Abstract
A lytic bacteriophage (phage), designated SAH-1, was isolated from sewage effluent near a dairy cow farm in Gwacheon, South Korea to search for biocontrol agents against Staphylococcus aureus infections. The SAH-1 was morphologically classified as Myoviridae and possessed an approximate 144 kb double-stranded genomic DNA. The phage showed broad host ranges within S. aureus strains including methicillin-resistant strains, and its latent period and burst size were approximately 20 min and 100 PFU/cell, respectively. Moreover, morphologic and genomic analysis of SAH-1 revealed that the phage was closely related to other Myoviridae phages infecting Staphylococcus species. The bacteriolytic activity of phage SAH-1 at a multiplicity of infection (MOI) 1 and 100 indicated its efficiency for reducing bacterial growth. Based on these results, phage SAH-1 could be considered a potential therapeutic or prophylactic candidate against S. aureus infections. (C) 2013 Elsevier Ltd. All rights reserved.
ISSN
0034-5288
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3091
DOI
10.1016/j.rvsc.2013.06.001
Bibliographic Citation
RESEARCH IN VETERINARY SCIENCE, v.95, no.2, pp.758 - 763, 2013
Publisher
ELSEVIER SCI LTD
Subject
BOVINE MASTITIS; MANAGEMENT; RESISTANCE; GENOME; GENE
Keywords
Bacteriophage; Mastitis; Myoviridae; SAH-1; Staphylococcus aureus
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCI LTD
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