Sequence-based genotyping methods to assess the genetic diversity of Riemerella anatipestifer isolates from ducklings with tremor SCIE SCOPUS

Cited 1 time in WEB OF SCIENCE Cited 1 time in Scopus
Title
Sequence-based genotyping methods to assess the genetic diversity of Riemerella anatipestifer isolates from ducklings with tremor
Author(s)
Han, Jee Eun; Kim, Ji Hyung; Choresca, Casiano, Jr.; Shin, Sang Phil; Jun, Jin Woo; Park, Se Chang
Publication Year
2013-10
Abstract
The aim of this study was to investigate sequence-based genotyping methods to distinguish 27 Riemerella anatipestifer isolates from ducklings in South Korea. The 16S rRNA sequences of the 27 R. anatipestifer isolates showed 99-100% similarities to each other and to reference sequences from Genbank (AY871822.2, AY871834.2, CP002562.1, EU715016.1, EU016548.1, EU715000.1, EU715008.1 and EU715011.1). In addition, the ompA gene sequences of 25 of the 27 R. anatipestifer isolates were 100% identical to each other, and these sequences were also 100% identical to reference sequences (CP002562.1, GQ415419.1, DQ059079, FJ765034.1, AY606207.1, AF104937.1, and FJ765033.1). Alternatively, four housekeeping genes (mdh, gdh, pgi, and rpoB) and three virulence-associated genes (prtC, hagA, and sspA) were used for a multi-locus sequence typing (MLST) and a single-locus sequence typing (SLST) among R. anatipestifer isolates. Compared to 16S rRNA and the ompA gene, seven genes showed higher genetic divergence patterns, and the isolates were separated into three distinct groups in phylogenetic trees.
ISSN
1121-7138
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3088
Bibliographic Citation
NEW MICROBIOLOGICA, v.36, no.4, pp.395 - 404, 2013
Publisher
EDIZIONI INT SRL
Subject
OUTER-MEMBRANE PROTEIN; COMPLETE GENOME SEQUENCE; ESCHERICHIA-COLI; OMPA; INFECTION; STRAIN; DUCKS
Keywords
Housekeeping genes; MLST; Phylogenetic analysis; Riemerella anatipestifer; SLST; Virulence-associated genes
Type
Article
Language
English
Document Type
Article
Publisher
EDIZIONI INT SRL
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