Extensive Isolation of Stress Responsive Genes in Soft Coral

Title
Extensive Isolation of Stress Responsive Genes in Soft Coral
Author(s)
염승식; 우선옥; 박홍석; 이택견
KIOST Author(s)
Yum, Seung Shic(염승식)Lee, Taek Kyun(이택견)
Publication Year
2006-11-06
Abstract
The coral reefs, including soft coral communities, are known to have critical importance for the marine ecosystem but are in serious decline mainly due to the global warming and human activities(1). The potentially thermal or chemical stress responsive genes from a soft coral Scleronephthya gracillimum were identified by construction of standard and stress exposed-paired subtractive cDNA library. The soft coral colonies were exposed to higher temperature (28°C) or exposed benzo[a]pyrene(2) (1 μM) for 24 hr under the controled condition. Three groups, including unexposed standard at 22°C, were subjected to synthesize cDNAs. The cDNA libraries of differentially expressed transcripts were generated by subtracting normal cDNA from the two kinds of stresses treated cDNA populations. During the first stage of the analysis, we have identified about 80 authentic candiates of differentially expressed genes to the thermal stress and benzo[a]pyrene exposure. The genes identified in this study have potential usefulness as biomarkers for monitoring the degree of environmental stresses on a soft coral species.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30772
Bibliographic Citation
2006 대한독성유전단백체학회 국제학술대회, pp.397, 2006
Publisher
대한독성유전단백체학회
Type
Conference
Language
English
Publisher
대한독성유전단백체학회
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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