Mitochondrial genomes of two jellyfishes, Aurelia aurita and Dactylometra quinquecirrha

Abstract

To get complete mitochondrial genomes from two jellyfishes, Aurelia aurita and Dactylometra quinquecirrha, we designed 14 conserved primer sets for amplifying the protein-coding region, 16S and 12S rRNAs. After amplification of these regions by polymerase chain reaction, we cloned them to pCR2.1 TA cloning vector for further analysis. Currently, we have sequenced 8 kb and 6 kb of two jellyfishes, A. aurita and D. quinquecirrha, respectively. These result may be applicable to designing the new markers to identify blooming-causing organisms, especially in early stages of development, and also be applicable to designing the specific primer sets for amplifying the complete mitogenome for other cryptic species of jellyfishes which is currently lacking