한국산 해양무척추동물의 DNA바코드 분석

Abstract

In the present study, we aimed the taxonomic revision of Korean marine invertebrates such as echinoderms, molluscs, annelida and sponges using the DNA barcoding system. We analyzed the invertebrates DNA barcode using the mitochondrial DNA sequences of cytochorome oxidase subunit I (COI) gene. After extraction of genomic DNA from the samples using QIAGEN kit, CO1 sequences were amplified by CO1 universal primer: COI universal primers (F: 5'- ggt caa caa atc ata aag ata ttg g - 3', R: 5'- taa act tca ggg tga cca aaa aat ca -3') recommended by Barcode of life. The amplified DNA fragments are sequenced and analysed by molecular phylogenetic method. We analyzed 36 species of the echinoderms, 30 of annelida, 10 of sponges and 9 of mollusca species. The Echinoderms including sea urchin, sea stars and sea cucumbers are a major group of marine invertebrates that can be found in almost every marine habitat. The Annelida (Polychaetas) are extremely variable in both morphology and lifestyle.