한국산 해양무척추동물의 DNA바코드 분석

Title
한국산 해양무척추동물의 DNA바코드 분석
Alternative Title
DNA BARCODE OF THE KOREAN MARINE INVERTEBRATES
Author(s)
김충곤; 정다금; 김고은; 남기웅; 박흥식; 배세진; 이윤호
KIOST Author(s)
Kim, Choong Gon(김충곤)Park, Heung-Sik(박흥식)Lee, Youn Ho(이윤호)
Publication Year
2007-09-18
Abstract
In the present study, we aimed the taxonomic revision of Korean marine invertebrates such as echinoderms, molluscs, annelida and sponges using the DNA barcoding system. We analyzed the invertebrates DNA barcode using the mitochondrial DNA sequences of cytochorome oxidase subunit I (COI) gene. After extraction of genomic DNA from the samples using QIAGEN kit, CO1 sequences were amplified by CO1 universal primer: COI universal primers (F: 5'- ggt caa caa atc ata aag ata ttg g - 3', R: 5'- taa act tca ggg tga cca aaa aat ca -3') recommended by Barcode of life. The amplified DNA fragments are sequenced and analysed by molecular phylogenetic method. We analyzed 36 species of the echinoderms, 30 of annelida, 10 of sponges and 9 of mollusca species. The Echinoderms including sea urchin, sea stars and sea cucumbers are a major group of marine invertebrates that can be found in almost every marine habitat. The Annelida (Polychaetas) are extremely variable in both morphology and lifestyle.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30365
Bibliographic Citation
The 2nd International Barcode of Life Conference, pp.111, 2007
Publisher
국제생물바코드컨소시엄
Type
Conference
Language
English
Publisher
국제생물바코드컨소시엄
Related Researcher
Research Interests

Marine Molecular Genetics,Marine Ecology and Evolution,해양분자유전,해양생태및진화

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