고세균 써모코커스 온누리누스 엔에이 원으로부터 히스티디놀 인산 분해 효소

DC Field Value Language
dc.contributor.author 이현숙 -
dc.contributor.author 조요나 -
dc.contributor.author 강성균 -
dc.contributor.author 이정현 -
dc.date.accessioned 2020-07-17T03:31:06Z -
dc.date.available 2020-07-17T03:31:06Z -
dc.date.created 2020-02-11 -
dc.date.issued 2007-09-23 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/30357 -
dc.description.abstract The entire gene cluster encoding enzymes involved in the histidine biosynthesis was analyzed in the complete genome of the hyperthermophilic archaeon Thermococcus onnurineus NA1 and it was found that an essential gene to encode the histidinol phosphate phosphatase (EC 3.1.3.15), catalyzing the eighth step of the pathway, was not assigned. A gene, which was annotated as a predicted hydrolase belonging to the haloacid dehalogenase superfamily, was speculated to specify the enzyme because it located next to the histidinol phosphate aminotransferase, which is a member of gene cluster. The gene was cloned and expressed in Escherichia coli. The recombinant enzyme exhibited Co2+-, Mg2+-, Mn2+-, or Ni2+-dependent phosphatase activity for AMP, phosphoserine, and fructose-6-phosphate among various substrates for other phosphatases. The implication of the gene to encode histidinol phosphate phosphatase was strongly supported by kinetic analysis. The catalytic efficiency for histidinol phosphate was two or three order of magnitude higher than those for the above three substrates. The orthologs were also observed in the other hyperthermophilic archaea, Thermococcus kodakaraensis (56.4% identity) and Pyrococcus furiosus. -
dc.description.uri 1 -
dc.language English -
dc.publisher Enzyme Engineering Conference -
dc.relation.isPartOf Enzyme Engineeing Conference -
dc.title 고세균 써모코커스 온누리누스 엔에이 원으로부터 히스티디놀 인산 분해 효소 -
dc.title.alternative A Novel Histidinol Phosphate Phosphatase, Catalyzing the Penulimate Step of the Histidine Biosynthesis Pathway, from a Hyperthermophilic Archaeon Thermococcus onnurineus NA1 -
dc.type Conference -
dc.citation.endPage 65 -
dc.citation.startPage 65 -
dc.citation.title Enzyme Engineeing Conference -
dc.contributor.alternativeName 이현숙 -
dc.contributor.alternativeName 조요나 -
dc.contributor.alternativeName 강성균 -
dc.contributor.alternativeName 이정현 -
dc.identifier.bibliographicCitation Enzyme Engineeing Conference, pp.65 -
dc.description.journalClass 1 -
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