Stepwise Involvement of Bacterial Strains During the Biofilm Formation

Abstract

Biofilm is a starting point of biofouling and/or biocorrosion in marine environment. However, only small information by multi-species is available. In the present study, properties of bacteria concerned in biofilm and interaction of selected bacteria during biofilm formation were investigated. Total 84 isolates from 3 days old biofilm could be assigned to 30 known genera which have been reported as widespread in marine environment. Approximately 31% of the isolates had been reported previously as uncultured or unidentified. Cell surface hydrophobicity and attachment ability were usually incompatible in a same strain and only 2 strains have both values high. Among the 39 strains produce Acyl-Homoserine Lactone (AHL), 21 strains could degrade AHL molecules. Strains belong to Actinobacteria displayed high cell surface hydrophobicity and Firmicutes displayed high attachment ability. In the continuous flow chamber system, growth-detachment- regrowth consist a cycle of biofilm process during 72 hours. It can be specified that, bacteria has high cell surface hydrophobicity plays major role at early stage and bacteria has high attachment attached ability plays after initial attachment.