Effects of heavy metals on transcription levels of antioxidant and stress responsive genes of Javanese medaka Oryzias javanicus

Title
Effects of heavy metals on transcription levels of antioxidant and stress responsive genes of Javanese medaka Oryzias javanicus
Author(s)
염승식; 우선옥
KIOST Author(s)
Yum, Seungshic(염승식)
Publication Year
2007-11-13
Abstract
The differential expression of eight genes representing stressor biomarkers (OjaCAT, OjaCYP1A, OjaG6PD, OjaGPx, OjaGR, OjaGST, OjaSOD, and OjaUB) was investigated by real-time quantitative PCR in liver of Javanese medaka, Oryzias javanicus, after exposure to Ag, Cd, Cu, Zn, Cr and Ni at various concentrations. The level of OjaCAT mRNA increased in a dose-dependent manner on 24-h exposure to Cd, Cu and Zn. However, after Ag, Cr and Ni exposure, a significant decrease in OjaCAT levels was observed. OjaCYP1A mRNA levels decreased drastically on exposure to all heavy metals tested. OjaG6PD mRNA levels showed a dramatic increment after 24-h exposure to Cu and Zn at low doses. However, mRNA levels decreased at high concentrations of Cu and Zn. In the case of Ag, Cd, Cr and Ni exposure, no prominent expression changes were observed. OjaGPx mRNA levels in liver were induced by Ag, Cd, Cu and Zn exposure, but suppressed by Cr and Ni exposure. Exposure to all heavy metals tested upregulated mRNA levels of OjaGR. OjaGST mRNA expression was upregulated by all heavy metals tested in a dose-dependent manner. OjaSOD mRNA levels increased on exposure to Ag, Cd, Zn and Cr, but no responses could be observed after Cu and Ni exposure. Positive induction of OjaUB expression was observed for all doses of heavy metal exposure. The induction and repression pattern of each gene level to the different heavy metals will provide multi-biomarker system for biomonitoring of aquatic environments.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30184
Bibliographic Citation
Society of Environmental Toxicology and Chemistry North America 28th Annual Meeting, pp.273 - 274, 2007
Publisher
Society of Environmental Toxicology and Chemistry North America
Type
Conference
Language
English
Publisher
Society of Environmental Toxicology and Chemistry North America
Related Researcher
Research Interests

Marine Genomics,Environmental Genomics,Novel biomaterials,해양생물유전체,생태환경유전체,바이오신소재

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse