한국해양생물의 DNA 바코드 분석

Title
한국해양생물의 DNA 바코드 분석
Alternative Title
DNA Barcoding of the Korean Marine Organisms
Author(s)
김충곤; 정다금; 김고은; 최은지; 이유철; Habib; 남기웅; 박흥식; 배세진; 이윤호
KIOST Author(s)
Kim, Choong Gon(김충곤)Park, Heung-Sik(박흥식)Lee, Youn Ho(이윤호)
Publication Year
2008-06-06
Abstract
We analyzed the Korean marine organisms DNA barcode using the mitochondrial DNA sequences of cytochorome oxidase subunit I (COI) gene. After extraction of genomic DNA from the samples using QIAGEN kit, COI sequences were amplified by COI universal primer: COI universal primers recommended by Barcode of life. The amplified DNA fragments are sequenced and analysed by molecular phylogenetic method. So far we analyzed 73 species of the marine fishes, 71 species of the echinoderms, 30 of annelida, 30 of sponges and 29 of mollusca species.The Echinoderms including sea urchin, sea stars and sea cucumbers are a major group of marine organisms that can be found in almost every marine habitat. The Annelida (Polychaetas) are extremely variable in both morphology and lifestyle. Sponges constitute a group of marine animals whose its morphological classification is very difficult because of a few morphological characters and a high degree of similarity. The COI barcode results showed that a significant differentiation between each species using this study except the sponge species. Mitochondrial DNA COI barcoding result shows homogeneity across some sponge species with scant variability and no geographical resolution. We also report the phylogenetic relationships of the organisms used in this study.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/29901
Bibliographic Citation
SMBE(Society for Molecular Biology and Evolution), 2008
Publisher
Annual Meeting of the Society for Molecular Biology and Evolution
Type
Conference
Language
English
Publisher
Annual Meeting of the Society for Molecular Biology and Evolution
Related Researcher
Research Interests

Marine Molecular Genetics,Marine Ecology and Evolution,해양분자유전,해양생태및진화

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