한국해양생물의 DNA 바코드 분석

Abstract

We analyzed the Korean marine organisms DNA barcode using the mitochondrial DNA sequences of cytochorome oxidase subunit I (COI) gene. After extraction of genomic DNA from the samples using QIAGEN kit, COI sequences were amplified by COI universal primer: COI universal primers recommended by Barcode of life. The amplified DNA fragments are sequenced and analysed by molecular phylogenetic method. So far we analyzed 73 species of the marine fishes, 71 species of the echinoderms, 30 of annelida, 30 of sponges and 29 of mollusca species.The Echinoderms including sea urchin, sea stars and sea cucumbers are a major group of marine organisms that can be found in almost every marine habitat. The Annelida (Polychaetas) are extremely variable in both morphology and lifestyle. Sponges constitute a group of marine animals whose its morphological classification is very difficult because of a few morphological characters and a high degree of similarity. The COI barcode results showed that a significant differentiation between each species using this study except the sponge species. Mitochondrial DNA COI barcoding result shows homogeneity across some sponge species with scant variability and no geographical resolution. We also report the phylogenetic relationships of the organisms used in this study.