한국에 서식하는 쥐노래미의 유전적 다양성과 집단구조 분석

Abstract

The Fat greenling(Hexagrammos otakii) is a commercial marine demersal fish in Korea as well as in East Asia. This species is one of the target fishes for the marine ranching program in Korea. Research on genetic diversity and population structure of the fat greenling is needed to acquire the basic data before any artificial breeding and marine ranching. For this purpose we anlyzed single nucleotide polymorphisms(SNPs) in the 697bp long partial sequences of the mtDNA COI region and the 764bp long sequences of CO3-ND3-ND4L region. In total of 139 individuals were analyzed; 50 from Tean in the West coast, 39 from Jumoonjin in the East coast and 50 from Tongyoung in the South coast. Twenty six variable sites comprising 27 different haplotypes were identified from the COI region and 35 variable sites comprising 33 different haplotypes from the CO3-Nd3-ND4L region. Comparison among the three populations for both genetic markers revealed that all three populations shared three common haplotypes with high frequency. The common haplotypes comprise approximately 70% of all the individuals from the three populations for both markers. These results indicate that the three populations are genetically closely related; separation of the populations was a recent event and/or gene flow is still occurring among the three populations. Haplotypes in the COI region can be grouped into three genetic lineage whereas the CO3-ND3-ND4L region can be grouped into four lineages. Some haplotypes are population specific so that they can be used for stock identification. In tern of genetic diversity, the Taean population is the most diverse among the three populations and the Jumoonjin population is the least diverse, which implies that the West coast population is the oldest one compared to the South and East coast populations.