Bacillus solimangrovi sp. nov., isolated from mangrove soil SCIE SCOPUS

Cited 15 time in WEB OF SCIENCE Cited 13 time in Scopus
Title
Bacillus solimangrovi sp. nov., isolated from mangrove soil
Author(s)
Lee, G.-H.; Rhee, M.-S.; Chang, D.-H.; Kwon, K.K.; Bae, K.S.; Yang, S.-H.; Kim, B.-C.
KIOST Author(s)
Kwon, Kae Kyoung(권개경)
Publication Year
2014
Abstract
Two novel bacterial strains, GH2-4T and GH2-5, were isolated from mangrove soil near the seashore of Weno island in Chuuk state, Micronesia, and were characterized by a polyphasic approach. The two strains were strictly aerobic, Gram-staining-positive, motile, endospore-forming rods that were catalase- and oxidase-positive. Colonies were circular, convex, stringy and transparent yellowish (GH2-4T) or opaque whitish (GH2-5). The 16S rRNA gene sequences of the two isolates were identical. The most closely related strains in terms of 16S rRNA gene sequence similarity were Bacillus kochii WCC 4582T, B. horneckiae DSM 23495T, B. azotoformans LMG 9581T, B. cohnii DSM 6307T and B. halmapalus DSM 8723T (95.6, 95.4, 95.4, 95.2 and 95.2% similarity, respectively). The partial groEL sequence of strain GH2-4T was identical to that of strain GH2-5 and showed <85% similarity to those of the most closely related strains. The isolates grew at pH 5-12 (optimal growth at pH 9), at 10-40 6C (optimum 30-35 6C) and at 0-9% (w/v) NaCl (optimum 1-3% NaCl). The cell-wall peptidoglycan of strains GH2-4T and GH2-5 contained meso-diaminopimelic acid and cell-wall hydrolysates contained ribose as a major sugar. The DNA G+C content was 36 mol%, and DNA-DNA relatedness between the isolates and five related reference strains was 20-24 %. Strain GH2-4T exhibited 81% DNA-DNA relatedness with strain GH2-5. The major cellular fatty acids of both strains were iso-C15: 0, iso-C16: 0, iso-C14:0 and anteiso-C15: 0 and the predominant menaquinone was MK-7. On the basis of the evidence from this polyphasic study, strains GH2-4T and GH2-5 (=KCTC 33143=JCM 18995=DSM 27084) represent a novel species of the genus Bacillus, for which the name Bacillus solimangrovi sp. nov. is proposed; the type strain is GH2-4T (=KCTC 33142T=JCM 18994T=DSM 27083T). © 2014 IUMS.
ISSN
1466-5026
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2951
DOI
10.1099/ijs.0.058230-0
Bibliographic Citation
International Journal of Systematic and Evolutionary Microbiology, v.64, no.PART 5, pp.1622 - 1628, 2014
Publisher
Society for General Microbiology
Subject
diaminopimelic acid; menaquinone; peptidoglycan; ribose; RNA 16S; article; Bacillus; Bacillus solimangrovi; bacterial cell wall; bacterial strain; DNA DNA hybridization; endospore-forming rods and cocci; gene sequence; mangrove; nonhuman; nucleotide sequence; priority journal; soil; Avicennia; Bacillus; Bacterial Typing Techniques; Base Composition; Chaperonin 60; Diaminopimelic Acid; DNA, Bacterial; Fatty Acids; Genes, Bacterial; Micronesia; Molecular Sequence Data; Nucleic Acid Hybridization; Peptidoglycan; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Vitamin K 2
Type
Article
Language
English
Document Type
Article
Publisher
Society for General Microbiology
Related Researcher
Research Interests

Acquisition of marine microbial bioresources,Marine microbial diversity,Application study on marine microbes,해양미생물 자원 확보,해양미생물 다양성,해양미생물 기능 활용

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