Comparison of CO-dependent H-2 production with strong promoters in Thermococcus onnurineus NA1 SCIE SCOPUS
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Seong Hyuk | - |
dc.contributor.author | Kim, Min-Sik | - |
dc.contributor.author | Bae, Seung Seob | - |
dc.contributor.author | Choi, Ae Ran | - |
dc.contributor.author | Lee, Jin-Won | - |
dc.contributor.author | Kim, Tae Wan | - |
dc.contributor.author | Lee, Jung-Hyun | - |
dc.contributor.author | Lee, Hyun Sook | - |
dc.contributor.author | Kang, Sung Gyun | - |
dc.date.accessioned | 2020-04-20T04:55:20Z | - |
dc.date.available | 2020-04-20T04:55:20Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2014-01 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2922 | - |
dc.description.abstract | To overproduce biotechnologically valuable products, the expression level of target genes has been modulated by using strong promoters. In a hyperthermophilic archaeon Thermococcus onnurineus NA1, two promoters, P (TN0413) and P (TN0157) , which drive expression of the genes encoding the S-layer protein and glutamate dehydrogenase were inserted in front of a gene cluster encoding a carbon monoxide dehydrogenase, a hydrogenase and a Na+/H+ antiporter. Two promoters exhibited strong activity by increasing the transcription and translation levels of the gene cluster in the mutant strains by 2.5- to 49-folds and 1.4- to 3.3-folds, respectively, than the native promoter in the wild-type strain. While KS0413 with P (TN0413) promoter exhibited 2.7 to 4.7 times higher transcript level than KS0157 with P (TN0157) promoter, the levels of proteins were a little different between them. The biomass concentrations and H-2 production rates of two mutants were 2- to 3-fold higher than those of the wild-type strain in a bioreactor where CO was supplied at a flow rate of 120 ml min(-1). Two mutants showed differential response to the higher CO flow rate, 240 ml min(-1), in terms of growth pattern and product formation, indicating two promoters were regulated by culture conditions. The results demonstrate that not only promoter strength but also product-forming conditions should be considered in promoter engineering. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | SEA HYDROTHERMAL VENT | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | GLUTAMATE-DEHYDROGENASE | - |
dc.subject | CONSTITUTIVE PROMOTERS | - |
dc.subject | BACTERIA | - |
dc.subject | CONSTRUCTION | - |
dc.subject | SEQUENCE | - |
dc.subject | ELEMENTS | - |
dc.subject | CLONING | - |
dc.title | Comparison of CO-dependent H-2 production with strong promoters in Thermococcus onnurineus NA1 | - |
dc.type | Article | - |
dc.citation.endPage | 986 | - |
dc.citation.startPage | 979 | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 98 | - |
dc.citation.number | 2 | - |
dc.contributor.alternativeName | 이성혁 | - |
dc.contributor.alternativeName | 김민식 | - |
dc.contributor.alternativeName | 배승섭 | - |
dc.contributor.alternativeName | 최애란 | - |
dc.contributor.alternativeName | 김태완 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.98, no.2, pp.979 - 986 | - |
dc.identifier.doi | 10.1007/s00253-013-5448-y | - |
dc.identifier.scopusid | 2-s2.0-84895068089 | - |
dc.identifier.wosid | 000329627600044 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | SEA HYDROTHERMAL VENT | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | GLUTAMATE-DEHYDROGENASE | - |
dc.subject.keywordPlus | CONSTITUTIVE PROMOTERS | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | CONSTRUCTION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | ELEMENTS | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordAuthor | Strong promoter | - |
dc.subject.keywordAuthor | Hyperthermophilic archaeon | - |
dc.subject.keywordAuthor | Carbon monoxide | - |
dc.subject.keywordAuthor | H-2 production | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |