해양생물의 활성도를 평가하기 위해서 fluorescein diacetate (FDA)와 Calcein-AM 방법 검토

Abstract

To evaluate whether marine phytoplankton species were rapidly able to apply on viability of living cells or not, the organism test was designed by staining methods of fluorescein diacetate (FDA) and Calcein-AM on each target species, such as bacillariphyceae, dinophyceae, raphidophyceae, chrysophyceae, haptophyceae and chlorophyceae. FDA method was the best dye for determining live cells of almost phytoplankton species, except several diatoms tested in this study. On the other hand, although fluorescence of Calcein-AM was very clear for a longer time, green fluorescence quantity per cell volume lacked in the tested almost species. In particular, gut of living zooplankton stained by Calcein-AM was strongly detected the green fluorescence than that of FDA staining cells or dead organisms. According to the Flow-CAM methods, green fluorescence values of stained cells by FDA were significantly higher than those of Calcein-AM treatments and control, implying that the Flow-CAM using FDA assay would adapt as important tool on the distinguish of living cells. Our results suggest that the FDA and Calcein-AM methods might be adapted to the phytoplankton and zooplankton, respectively, though species-specific characters are greatly different by organisms.