Anti-inflammatory Activity of Sulfated Polysaccharide Purified from AMG Extract of Ecklonia cava

Abstract

Ecklonia cava has reported anti-thrombotic, immunomodulatory and anti-inflammatory effects in vitro and in vivo. We evaluated the potential activity of the derivates such as the sulfated polysaccharide from E. cava on anti-inflammatory activity in LPS-stimulated RAW 264.7 cell. In this study, E. cava was collected from Jeju Island of Korea and hydrolyzed by food grade and less expensive carbohydrases and proteases. We showed the AMG digest, which is the product hydrolyzed by AMG, has the highest inhibitory effects on NO production in LPS-stimulated RAW 264.7 cells. To identify the active compounds from the AMG digest, we performed the molecular membrane system, the ethanol separation, the anion exchange and gel permeation chromatography, and the 50kDa over fraction, the crude polysaccharide (CPS) and the purified polysaccharide (PPA) were obtained. The AMG digests’ derivates (50kDa over fraction, CPS and PPA) significantly inhibited the NO production and PPA showed the highest inhibitory activity on NO production. Also, PPA decreased the prostaglandin-E2 (PGE2) production and suppressed the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in the LPS-stimulated Raw 264.7 cells. Thus, PPA inhibited NO and PGE2 production through the inhibition of iNOS and COX-2 expression in LPS-stimulated RAW 264.7. These result suggest that the anti-inflammatory activity of PPA may be due to modulation of anti-inflammatory agent.