Anti-inflammatory Activity of Sulfated Polysaccharide Purified from AMG Extract of Ecklonia cava

Title
Anti-inflammatory Activity of Sulfated Polysaccharide Purified from AMG Extract of Ecklonia cava
Author(s)
Sang-Won Han; Sung-Myung Kang; 허수진; Ki-Wan Lee; You-Jin Jeon
KIOST Author(s)
Heo, Soo Jin(허수진)
Publication Year
2010-04-29
Abstract
Ecklonia cava has reported anti-thrombotic, immunomodulatory and anti-inflammatory effects in vitro and in vivo. We evaluated the potential activity of the derivates such as the sulfated polysaccharide from E. cava on anti-inflammatory activity in LPS-stimulated RAW 264.7 cell. In this study, E. cava was collected from Jeju Island of Korea and hydrolyzed by food grade and less expensive carbohydrases and proteases. We showed the AMG digest, which is the product hydrolyzed by AMG, has the highest inhibitory effects on NO production in LPS-stimulated RAW 264.7 cells. To identify the active compounds from the AMG digest, we performed the molecular membrane system, the ethanol separation, the anion exchange and gel permeation chromatography, and the 50kDa over fraction, the crude polysaccharide (CPS) and the purified polysaccharide (PPA) were obtained. The AMG digests’ derivates (50kDa over fraction, CPS and PPA) significantly inhibited the NO production and PPA showed the highest inhibitory activity on NO production. Also, PPA decreased the prostaglandin-E2 (PGE2) production and suppressed the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in the LPS-stimulated Raw 264.7 cells. Thus, PPA inhibited NO and PGE2 production through the inhibition of iNOS and COX-2 expression in LPS-stimulated RAW 264.7. These result suggest that the anti-inflammatory activity of PPA may be due to modulation of anti-inflammatory agent.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/29009
Bibliographic Citation
제24회 한국조류학회 정기학술발표회, pp.126, 2010
Publisher
한국조류학회
Type
Conference
Language
English
Publisher
한국조류학회
Related Researcher
Research Interests

Marine Biotechnology,Marine Natural Products,Bioactivities,해양생물공학,해양천연물학,생리활성

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse