Effect of fucoxanthin on the MAPKs signaling pathway and induction of apoptosis in B16F10 melanoma cells

Title
Effect of fucoxanthin on the MAPKs signaling pathway and induction of apoptosis in B16F10 melanoma cells
Author(s)
Sung-Myung Kang; Kil-Nam Kim; Won-Woo Lee; 허수진; Ki-Wan Lee; You-Jin Jeon
KIOST Author(s)
Heo, Soo Jin(허수진)
Publication Year
2010-04-29
Abstract
Fucoxanthin, a natural carotenoid, has been reported to have antitumorigenic activity in mouse colon, skin and duodenum models. The present study was designed to evaluate the molecular mechanisms of fucoxanthin against melanoma cell lines (B16F10 cells). Fucoxanthin reduced the viability of B16F10 cells in a dose-dependent manner accompanied by the induction of cell cycle arrest during the G0/G1 phase and apoptosis. Fucoxanthin-induced G0/G1 arrest was associated with a marked decrease in the protein expressions of phosphorylated-Rb (retinoblastoma protein), cyclin D(1 and 2) and cyclin-dependent kinase (Cdk)4 and up-regulation of the protein levels of p15INK4B and p27Kip1. Fucoxanthin-induced apoptosis was accompanied with down-regulation of the protein levels of Bcl-xL, and inhibitor of apoptosis protein (XIAP), resulting in cytochrome c release and sequential activation of caspase-9, caspase-3, and PARP. Furthermore, fucoxanthin activated c-Jun N-terminal kinase (JNK), p38 MAPK (p38) and extracellular signal-regulated kinase (ERK) on B16F10 cells in a time-dependent manner. These results suggest that fucoxanthin has anti-tumor properties on melanoma cells by inducing selectively the genes related to cell cycle arrest and apoptosis.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/29007
Bibliographic Citation
제24회 한국조류학회 정기학술발표회, pp.131, 2010
Publisher
한국조류학회
Type
Conference
Language
English
Publisher
한국조류학회
Related Researcher
Research Interests

Marine Biotechnology,Marine Natural Products,Bioactivities,해양생물공학,해양천연물학,생리활성

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