Pyrene metabolism and enzymes involved in Novosphingobium pentaromativorans US6-1

Title
Pyrene metabolism and enzymes involved in Novosphingobium pentaromativorans US6-1
Author(s)
Yuanrong Luo; 윤성호; 권개경; 김상진; 김승일; 정영호
KIOST Author(s)
Kwon, Kae Kyoung(권개경)
Publication Year
2010-05-07
Abstract
Pyrene is a polycyclic aromatic hydrocarbon (PAH) commonly present in PAH-contaminated sites. Novosphingobium pentaromativorans US6-1 was isolated from sediment of Ulsan bay and has broad substrates with two to five rings PAHs. Newly and increased expression of proteins were found with 1-D PAGE or 2-DE and LC-MS/MS. Presence of pyrene 1,2-monoxygenase, pyrene 4,5-monoxygenase and 4,5-dihydroxypyrene dioxygenase indicates that degradation of pyrene in N. pentaromativorans US6-1 proceeds via multiple metabolic routes initiated by mono-(C-1,2 and C-4,5) and dioxygenation (C-4,5) reactions. Further degradation entered phenanthrene metabolic pathway and via either o-phthalate pathway or salicylate pathway, both pathways were subsequently entered tricarboxylic acid (TCA) cycle and mineralized to CO2. A pyrene degradation pathway for N. pentaromativorans US6-1 was proposed by identifying the enzymes involved in the pyrene degradation.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/28990
Bibliographic Citation
2010 한국미생물학회 국제학술대회, pp.125, 2010
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
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