Pyrene metabolism and enzymes involved in Novosphingobium pentaromativorans US6-1
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Title
- Pyrene metabolism and enzymes involved in Novosphingobium pentaromativorans US6-1
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Author(s)
- Yuanrong Luo; 윤성호; 권개경; 김상진; 김승일; 정영호
- KIOST Author(s)
- Kwon, Kae Kyoung(권개경)
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Alternative Author(s)
- 권개경; 김상진
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Publication Year
- 2010-05-07
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Abstract
- Pyrene is a polycyclic aromatic hydrocarbon (PAH) commonly present in PAH-contaminated sites. Novosphingobium pentaromativorans US6-1 was isolated from sediment of Ulsan bay and has broad substrates with two to five rings PAHs. Newly and increased expression of proteins were found with 1-D PAGE or 2-DE and LC-MS/MS. Presence of pyrene 1,2-monoxygenase, pyrene 4,5-monoxygenase and 4,5-dihydroxypyrene dioxygenase indicates that degradation of pyrene in N. pentaromativorans US6-1 proceeds via multiple metabolic routes initiated by mono-(C-1,2 and C-4,5) and dioxygenation (C-4,5) reactions. Further degradation entered phenanthrene metabolic pathway and via either o-phthalate pathway or salicylate pathway, both pathways were subsequently entered tricarboxylic acid (TCA) cycle and mineralized to CO2. A pyrene degradation pathway for N. pentaromativorans US6-1 was proposed by identifying the enzymes involved in the pyrene degradation.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/28990
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Bibliographic Citation
- 2010 한국미생물학회 국제학술대회, pp.125, 2010
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Publisher
- 한국미생물학회
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Type
- Conference
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Language
- English
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