EFFECTS OF THE IRANIAN HEAVY CRUDE OIL ON THE EARLY LIFE STAGES OF ZEBRA FISH (DANIO RERIO)

Title
EFFECTS OF THE IRANIAN HEAVY CRUDE OIL ON THE EARLY LIFE STAGES OF ZEBRA FISH (DANIO RERIO)
Author(s)
정지현; J.P. Incardona; T.K.Collier; 심원준
KIOST Author(s)
Jung, Jee Hyun(정지현)Shim, Won Joon(심원준)
Publication Year
2010-06-02
Abstract
These studies were prompted by the Hebei Spirit oil spill examined and the effects of 12,547 kl of crude oil which were spilled from the tanker into the west coast of Korea. Three different kinds of crude oils (Iranian Heavy, KEC and U/Zkum) were spilled from the punctured tanks. To clarify the effects of the spilled oil on fish early life stages, A laboratory study was conducted using zebrafish embryos. Zebrafish embryos (96 h post-fertilization) were exposed to gravel containing Iranian Heavy Crude (IHC) oil at a concentration of 6g/kg for 72h. Embryos were manually dechorionated and observed by a stereomicroscope and a compound microscope. Embryos were fixed in 4% phosphate-buffered paraformaldehyde and stained for CYP1A and myosin heavy chain. After 48 hr, several complex effects, including the edema and tail mutation were observed. 65% and 80% of embryos showed pericardiac edema and tail abnormality, respectively. About 90% of embryos showed pericardiac edema and tail abnormality after 72hr. 19 PAHs and alkylated PAHs concentrations in water were measured. Naphthalene dominated in oiled gravel. Tricyclic PAHs were accounted 82-89% of 19 PAHs and alkylated PAHs accounted for 16-27. The distribution of CYP1A in embryo was assessed by immunofluorescence. CYP1A immunofluorescence activity was visible in the endothelium of head, the epidermis and the endocardium. Our results confirm that Zebra fish embryos exposed to IHC may suffer disrupted cardiac function and fish tail during development both of which are highly relevant for fitness and survival.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/28953
Bibliographic Citation
6th International conference on marine pollution and ecotoxicology, pp.126, 2010
Publisher
City Univ. of Hong Kong
Type
Conference
Language
English
Publisher
City Univ. of Hong Kong
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

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