Identification of Ruditapes philippinarum and Meretrix lusoria Larvae Using Single Cell PCR Analysis and Microscopic Observation

Title
Identification of Ruditapes philippinarum and Meretrix lusoria Larvae Using Single Cell PCR Analysis and Microscopic Observation
Author(s)
정승원; 김영옥; 이진환
KIOST Author(s)
Jung, Seung Won(정승원)Kim, Young Ok(김영옥)
Publication Year
2010-06-18
Abstract
To identify free living bivalve larvae in the coastal waters of Taean marine ranch of Korea, single cell PCR analysis and light and scanning electron microscopic observation were applied. Isolated two venerid clam larvae by DNA sequencing were identified with Ruditapes philippinarum (99% of similarity) and Meretrix lusoria (99%). In microscopic observation, R. philippinarum in a D-veliger stage had symmetrical shoulder angles and an elliptical ventral form, in contrast to the asymmetrical shoulder angles and a round ventral form of M. lusoria in an umbonal stage. Size of the R. philippinarum larva was 156 ± 22 μm in length, 126 ± 12 μm in height, 92 ± 14 μm in width and 1.23 in ratio of length vs. height. M. lusoria was 202 ± 44 μm in length, 161 ± 35 μm in height, 96 ± 38 μm in width and 1.25 in ratio of length vs. height. These results indicate that for two venerid clam larvae, the characteristics in morphological and molecular information provide the evidence for larval identification in a nature.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/28830
Bibliographic Citation
한국환경생물학회 춘계 학술대회, pp.49, 2010
Publisher
한국환경생물학회
Type
Conference
Language
English
Publisher
한국환경생물학회
Related Researcher
Research Interests

Marine ecology,Plankton,Protists,해양생태,부유생물,원생생물

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse