Evaluation of sediment pollution in waste dumping sites using polychaete, Perinereis aibuhitensis

Evaluation of sediment pollution in waste dumping sites using polychaete, Perinereis aibuhitensis
원효경; 우선옥; 전혜영; 김보라; 염승식
KIOST Author(s)
Won, Hyokyoung(원효경)Woo, Seon Ock(우선옥)Yum, Seung Shic(염승식)
Publication Year
The aim of this study is the investigating the biological effects of marine sediment layer in waste dumping sites on the sediment-dwelling organisms. The marine sediments were collected from the reference sites (2), dumping sites (2), the past dumping site (1) and the past red soil dumping area (1) in the East Sea. The pore waters were prepared from the 6 sediment samples and a polychaete, Perinereis aibuhitensis was exposed to the 6 pore waters, control seawater and 200 ppb of CdCl2 for 24 hours, respectively. RNAs were extracted from the individuals and the gene expressions of metal binding proteins, ferritin (FER) and hemerythrin-like metalloprotein (HLM) were analyzed by quantitative RT-PCR using the gene specific primers. As a result, both metal involved genes expressions increased 2.1~3.1-fold (FER) and 1.5~2.4-fold (HLM) respectively in the CdCl2-exposed group, the current dumping site group (site 26) and the past dumping site group (site130) comparing with control group. And both genes were down-regulated over 1.7-fold in the past red soil dumping site group (site 79) comparing with control group. FER expression was up-regulated 1.4~2.1-fold in the CdCl2-exposed individuals, site 26, and site 130 groups comparing with reference groups. FER gene in the control group and site 79 group was expressed 1.5 and 2.5-fold less than reference groups. Comparing with CdCl2 exposed group, two genes expressions were similar to site 26. Summarizing the results, two metal involved gene expressions were up-regulated in current dumping sites and a post-dumping site comparing with each control and reference groups. In conclusion, transcriptional changes of specific genes could be a good indicator for assessing contamination of sediment layers and the exploration of specific gene biomarkers can be more helpful to explain the various effects induced by polluted sediment.
Bibliographic Citation
3rd International Conference on Environmental Health Science, pp.p019, 2010
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