Ferritin and hemerythrin-like metalloprotein gene expressions in a polychaete, Perinereis aibuhitensis exposed to polluted sediment

Ferritin and hemerythrin-like metalloprotein gene expressions in a polychaete, Perinereis aibuhitensis exposed to polluted sediment
원효경; 우선옥; 전혜영; 김보라; 염승식
KIOST Author(s)
Yum, Seungshic(염승식)
Publication Year
This study aimed to examine the biological effects of marine sediment layer in waste dumping sites on the sediment-dwelling organisms. The marine sediments were collected from the reference sites (2), dumping sites (2), the past dumping site (1) and the past red soil dumping area (1) in the East Sea. The pore waters were prepared from the 6 sediment samples and a polychaete, Perinereis aibuhitensis was exposed to the 6 pore waters, control seawater and 200 ppb of CdCl2 for 24 hours, respectively. RNAs were extracted from the exposed individuals and the gene expressions of metal binding proteins, ferritin (FER) and hemerythrin-like metalloprotein (HLM) were analyzed by quantitative RT-PCR using the gene specific primers. As expected the exposure to CdCl2 changed both of metal involved genes expressions up-regulated (3.2 and 2.0-fold) comparing with control group and specifically FER gene expressed over 2-fold higher in CdCl2 -exposed group than both control and reference sites groups. In current dumping sites FER gene expressions were significantly higher than the control and reference sites samples. In a current dumping site (site 26) the both gene expressions increased 2.9-fold and 2.0-fold comparing with the control. The gene expressions of FER and HLM decreased in the past-red soil dumping site (site 79) but increased over 1.5-fold in the other past dumping site. Summarizing the results, two metal involved gene expressions were up-regulated in current dumping sites and a post-dumping site comparing with control group and especially FER gene responded significantly in all dumping sites. In conclusion, transcriptional changes of specific genes could be a good indicator for assessing contamination of sediment layers and the exploration of specific gene biomarkers can be more helpful to explain the various effects induced by polluted sediment.
Bibliographic Citation
6th International Conference on Toxicogenomics 2010, pp.37, 2010
Related Researcher
Research Interests

Marine Genomics,Environmental Genomics,Novel biomaterials,해양생물유전체,생태환경유전체,바이오신소재

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