Protective Effect of brown seaweeds on UVB-induced Oxidative Stress, in vitro and in vivo zebrafish model
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Title
- Protective Effect of brown seaweeds on UVB-induced Oxidative Stress, in vitro and in vivo zebrafish model
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Author(s)
- Seok-Chun Ko; 허수진; Seung-Hong Lee; Sung-Myung Kang; Seon-Heui Cha; You-Jin Jeon
- KIOST Author(s)
- Heo, Soo Jin(허수진)
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Alternative Author(s)
- 허수진
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Publication Year
- 2010-11-18
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Abstract
- Chronic exposure of the skin to ultraviolet B (UVB) radiation induces oxidative stress, which plays a crucial role in the induction of skin aging. In this study, the protective effect of extracts of the brown seaweed Ecklonia cava on UVB radiation-induced oxidative stress was assessed in viability tests measuring the intracellular fluorescence of dichlorofluorescein diacetate and colorimetric reduction of 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide, and Hoechst 33342/propidium iodide staining of apoptosis activity. E. cava extracts evidenced a profound effect against UVB radiation-induced oxidative stress. The highest activity was produced by the 100% methanol extract. Partitioning of the methanol extract with different organic solvents revealed a pronounced protective effect in the ethyl acetate fraction. The isolated active compounds were phlorotannins, especially dieckol, as identified using ultraperformance liquid chromatography-tandem mass spectrometry. Among the phlorotannins, dieckol showed a higher protective effect than the other phlorotannins. Therefore, UVB protection of dieckol was evaluated via DCFH-DA, DAF-FM DA, acridine orange and morphological changes in zebrafish. Hence, these results indicated that dieckol has potential protective effects on UVB-induced cell death, which might be useful in antioxidant and cosmetic formulations.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/28519
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Bibliographic Citation
- The 6th KSMB Annual Meeting and International Mini-symposium, pp.59, 2010
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Publisher
- Korean Society of Marine Biotechnology
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Type
- Conference
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Language
- English
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