Development of a rapid and effective method for preparing delicate dinoflagellates for scanning electron microscopy
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Title
- Development of a rapid and effective method for preparing delicate dinoflagellates for scanning electron microscopy
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Alternative Title
- Development of a rapid and effective method for preparing delicate dinoflagellates for scanning electro
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Author(s)
- 정승원; 주형민; 이진환; 임동일
- KIOST Author(s)
- Jung, Seung Won(정승원); Lim, Dhong Il(임동일)
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Alternative Author(s)
- 정승원; 임동일
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Publication Year
- 2011-09-06
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Abstract
- We developed a rapid and effective procedure for scanning electron microscopy of three delicate dinoflagellates, Karlodinium micrum, Akashiwo sanguinea and Heterocapsa niei. Good results were obtained when the specimens were fixed with a modified Párducz’s fixative (2% osmium tetroxide:saturated mercuric chloride=5:1 v/v) for 10 min, washed in 0.05 M sodium cacodylate trihydrate buffer for 2 min, dehydrated in a tert-butanol for 10 min and dried with hexamethyldisilazane in air for 3 min. All reagents were carefully used in a fume hood because reagents are very toxic. This method could be completed in 25 min. Compared with other preparative techniques, the present protocol has significant advantages for SEM observation by limiting distortion of delicate specimens and reducing the preparation time.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/28221
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Bibliographic Citation
- IX International Conference on Modern and Fossil Dinoflagellates, pp.53, 2011
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Publisher
- IX International conference on modern and fossil dinoflagellates
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Type
- Conference
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Language
- English
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