Chromene suppresses the activation of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells SCIE SCOPUS

Cited 21 time in WEB OF SCIENCE Cited 22 time in Scopus
Title
Chromene suppresses the activation of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells
Author(s)
Heo, Soo-Jin; Jang, Jiyi; Ye, Bo-Ram; Kim, Min-Sun; Yoon, Weon-Jong; Oh, Chulhong; Kang, Do-Hyung; Lee, Ji-Hyeok; Kang, Min-Cheol; Jeon, You-Jin; Kang, Sung-Myung; Kim, Daekyung; Kim, Kil-Nam
KIOST Author(s)
Heo, Soo Jin(허수진)Oh, Chulhong(오철홍)Kang, Do-Hyung(강도형)
Publication Year
2014-05
Abstract
Inflammation is complex process involving a variety of immune cells that defend the body from harmful stimuli. However, pro-inflammatory cytokines and inflammatory mediators can also exacerbate diseases such as cancer. The aim of this study was to identify a natural effective remedy for inflammation. We isolated a functional algal chromene compound from Sargassum siliquastrum, named sargachromanol D (SD). We evaluated the anti-inflammatory effect of SD on lipopolysaccharide (LPS)-exposed RAW 264.7 cells by measuring cell viability, cytotoxicity, and production of inflammatory mediators such as nitric oxide (NO), prostaglandin E-2 (PGE(2)), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and the pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta, and IL-6. SD inhibited production of NO and PGE(2) from LPS-induced cells by preventing the expression of inflammatory mediators such as iNOS and COX-2 in a dose-dependent manner. Concurrently, levels of the pro-inflammatory cytokines TNF-alpha, IL-1 beta, and IL-6 were reduced with increasing concentrations of SD. In addition, SD inhibited the activation of NF-kappa B and mitogen-activated protein kinases (MAPKs) pathways in a concentration-dependent manner. These restilts indicate that SD inhibits LPS-stimulated inflammation by inhibition of the NF-kappa B and MAPKs pathways in macrophages. (C) 2014 Elsevier Ltd. All rights reserved.
ISSN
0278-6915
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2821
DOI
10.1016/j.fct.2014.02.023
Bibliographic Citation
FOOD AND CHEMICAL TOXICOLOGY, v.67, pp.169 - 175, 2014
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Subject
NITRIC-OXIDE SYNTHASE; NF-KAPPA-B; SIGNAL-REGULATED KINASE; SARGASSUM-SILIQUASTRUM; PROINFLAMMATORY CYTOKINES; MURINE MACROPHAGES; PROTEIN-KINASE; EXPRESSION; CANCER; INHIBITION
Keywords
Sargassum siliquastrum; Anti-inflammation; Inflammatory mediators; Pro-inflammatory cytokines; Chromene
Type
Article
Language
English
Document Type
Article
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
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