Chromene suppresses the activation of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells
SCIE
SCOPUS
Cited 27 time in
WEB OF SCIENCE
Cited 28 time in
Scopus
-
Title
- Chromene suppresses the activation of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells
-
Author(s)
- Heo, Soo-Jin; Jang, Jiyi; Ye, Bo-Ram; Kim, Min-Sun; Yoon, Weon-Jong; Oh, Chulhong; Kang, Do-Hyung; Lee, Ji-Hyeok; Kang, Min-Cheol; Jeon, You-Jin; Kang, Sung-Myung; Kim, Daekyung; Kim, Kil-Nam
- KIOST Author(s)
- Heo, Soo Jin(허수진); Oh, Chul Hong(오철홍); Kang, Do Hyung(강도형)
-
Alternative Author(s)
- 허수진; 장지이; 예보람; 김민선; 오철홍; 강도형
-
Publication Year
- 2014-05
-
Abstract
- Inflammation is complex process involving a variety of immune cells that defend the body from harmful stimuli. However, pro-inflammatory cytokines and inflammatory mediators can also exacerbate diseases such as cancer. The aim of this study was to identify a natural effective remedy for inflammation. We isolated a functional algal chromene compound from Sargassum siliquastrum, named sargachromanol D (SD). We evaluated the anti-inflammatory effect of SD on lipopolysaccharide (LPS)-exposed RAW 264.7 cells by measuring cell viability, cytotoxicity, and production of inflammatory mediators such as nitric oxide (NO), prostaglandin E-2 (PGE(2)), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and the pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta, and IL-6. SD inhibited production of NO and PGE(2) from LPS-induced cells by preventing the expression of inflammatory mediators such as iNOS and COX-2 in a dose-dependent manner. Concurrently, levels of the pro-inflammatory cytokines TNF-alpha, IL-1 beta, and IL-6 were reduced with increasing concentrations of SD. In addition, SD inhibited the activation of NF-kappa B and mitogen-activated protein kinases (MAPKs) pathways in a concentration-dependent manner. These restilts indicate that SD inhibits LPS-stimulated inflammation by inhibition of the NF-kappa B and MAPKs pathways in macrophages. (C) 2014 Elsevier Ltd. All rights reserved.
-
ISSN
- 0278-6915
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2821
-
DOI
- 10.1016/j.fct.2014.02.023
-
Bibliographic Citation
- FOOD AND CHEMICAL TOXICOLOGY, v.67, pp.169 - 175, 2014
-
Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
-
Subject
- NITRIC-OXIDE SYNTHASE; NF-KAPPA-B; SIGNAL-REGULATED KINASE; SARGASSUM-SILIQUASTRUM; PROINFLAMMATORY CYTOKINES; MURINE MACROPHAGES; PROTEIN-KINASE; EXPRESSION; CANCER; INHIBITION
-
Keywords
- Sargassum siliquastrum; Anti-inflammation; Inflammatory mediators; Pro-inflammatory cytokines; Chromene
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.