A rapid and effective method for preparing delicate dinoflagellates for scanning electron microscopy
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Title
- A rapid and effective method for preparing delicate dinoflagellates for scanning electron microscopy
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Author(s)
- 정승원; 임동일
- KIOST Author(s)
- Jung, Seung Won(정승원); Lim, Dhong Il(임동일)
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Alternative Author(s)
- 정승원; 임동일
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Publication Year
- 2011-10-14
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Abstract
- We developed a rapid and effective procedure for scanning electron microscopy of three delicate dinoflagellates, Karlodinium micrum, Akashiwo sanguinea and Heterocapsa niei. Good results were obtained when the specimens were fixed with a modified Párducz’s fixative (2% osmium tetroxide:saturated mercuric chloride=5:1 v/v) for 10 min, washed in 0.05 M sodium cacodylate trihydrate buffer for 2 min, dehydrated in a tert-butanol for 10 min and dried with hexamethyldisilazane in air for 3 min. All reagents should be carefully used because the reagents are very toxic. This method could be completed in 25 min. Compared with other preparative techniques, the present protocol has significant advantages for SEM observation by limiting distortion of delicate specimens and reducing the preparation time.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/28173
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Bibliographic Citation
- 6th Asian Pacific Phycological forum, pp.373, 2011
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Publisher
- The Korean Society of Phycology
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Type
- Conference
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Language
- English
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