Hoppeia youngheungensis gen. nov., sp nov., a member of the Flavobacteriaceae isolated from tidal flat sediment, and emended descriptions of the genus Sediminibacter and Sediminibacter furfurosus

Abstract

A Gram-reaction-negative, proteorhodopsin-containing, yellow-pigmented, rod-shaped, non-gliding and strictly aerobic bacterium, designated strain YIK12(T), was isolated from tidal flat sediment of Yeongheung Island at the coast of the West Sea of Korea. Cells produced non-diffusible carotenoid pigments, but not flexirubin-type pigment. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate was affiliated to the family Flavobacteriaceae and showed highest similarity to Sediminibacter furfurosus MAOS-86(T) (93.3%). Growth was observed at 24-44 degrees C (optimum 30 degrees C), at pH 6.5-8.0 (optimum 7.0) and in the presence of 1.5-7.0% sea salts (optimum 2%). The DNA G+C content was 40.4 mol% and the predominant cellular fatty acids were iso-C-15:0, iso-C-15:1 G, iso-C-17:0 3-OH, iso-C-16:0 3-OH, anteiso-C-15:0 and iso-C-(15:0) 3-OH. The major respiratory quinone was MK-6. On the basis of data from the present polyphasic taxonomic study, strain YIK12(T) is considered to represent a novel species of a new genus in the family Flavobacteriaceae, for which the name Hoppeia youngheungensis gen. nov., sp. nov. is proposed. The type strain of H. youngheungensis is YIK12(T) (=KCCM 43023(T)=JCM 19488(T)). Emended descriptions of the genus Sediminibacter and Sediminibacter furfurosus are given.