Macrolactin Antibiotics from a Marine-derived bacterium Bacillus sp. 102CH635-3

Abstract

The macrolactins are a group of 24-membered lactones generally isolated from the deep sea bacteria, Actinomadura sp. and Bacillus sp.. The strain Bacillus sp. 102CH635-3 was isolated from a marine sediment sample collected from mear Chuuck state in the Federated states of Micronesia. Compounds 1-4 were purified from the EtOAc extract, obtained from the culture broth of the strain 102CH635-3, by repeated chromatographic steps. Several studies have suggested that most macrolactins have potent bioactivities, such as antibacterial, cytotoxic and antiviral activities. The chemical structures of 1-4 were elucidated on the basis of MS, 1D and 2D NMR spectroscopic data analysis and literature review. Macrolactins A(1), F(2), I(3), and R(4) showed antibacterial, cytotoxic and antiviral activities. Here we present the production, isolation and structure determination of macrolactin 1-4.