New Macrolactins as Antibiotics Produced by the Marine-derived Bacterium Bacillus sp. 102CH635-3

Abstract

Marine microorganisms live in a quite different environment from their terrestrial counterparts and would thus be expected to have a different metabolic pathway and to produce compounds which prossess unique structures and activities. The macrolactins are a group of 24-membered lactones generally isolated from the deep sea bacteria1), Actinomadura sp.2) and Bacillus sp.3-5) The strain Bacillus sp. 102CH635-3 was isolated from a marine sediment sample collected from near Chuuk state in the Federated states of Micronesia(Fig. 1). Compounds 1-6 were purified from the EtOAc extract, obtained from the culture broth of the strain 102CH635-3(Fig. 2), by repeated choromatographic steps. Several studies have suggested that most macrolactins have potent bioactivities, such as antibacterial, cytotoxic and antiviral activities. The chemical stractures of 1-6 were elucidated on the basis of MS, 1D and 2D NMR spectroscopic data analysis and literature review. The macrolactins are a group of 24-membered lactones generally isolated from the deep sea bacteria1), Actinomadura sp.2) and Bacillus sp.3-5) The strain Bacillus sp. 102CH635-3 was isolated from a marine sediment sample collected from near Chuuk state in the Federated states of Micronesia(Fig. 1). Compounds 1-6 were purified from the EtOAc extract, obtained from the culture broth of the strain 102CH635-3(Fig. 2), by repeated choromatographic steps. Several studies have suggested that most macrolactins have potent bioactivities, such as antibacterial, cytotoxic and antiviral activities. The chemical stractures of 1-6 were elucidated on the basis of MS, 1D and 2D NMR spectroscopic data analysis and literature review.