Differentially expressed genes in perfluorooctane sulfonate exposed Hydra

Abstract

We evaluated perfluorooctane sulfonate (PFOS)-induced acute toxicity in Hydra magnipapillata. The median lethal concentration of the animals (LC50) were determined to be 71.0 mg/L and 27.2 mg/L after exposure to PFOS for 24 h and 48 h, respectively. The morphological responses of hydra polyps to a range of exposure time were illustrated. To define the repertoire of Hydra genes responding to acute exposure of PFOS, transcriptome dynamics were examined in a series of exposure time (4 h, 12 h, 24 h, and 48 h) by using DNA microarray which contains about 18,000 singletons. As the results, we obtained 216, 103, 881, and 659 differentially expressed gene candidates response to PFOS (300 μg/L) exposure for 4 h, 12 h, 24 h and 48 h, respectively. The functional analysis of these candidate genes is underway. These candidate genes have great potential in use as multi-biomarkers for organic pollutants and provide a clue in mechanistic aspects of PFOS toxicity in aquatic animals. respectively. The morphological responses of hydra polyps to a range of exposure time were illustrated. To define the repertoire of Hydra genes responding to acute exposure of PFOS, transcriptome dynamics were examined in a series of exposure time (4 h, 12 h, 24 h, and 48 h) by using DNA microarray which contains about 18,000 singletons. As the results, we obtained 216, 103, 881, and 659 differentially expressed gene candidates response to PFOS (300 μg/L) exposure for 4 h, 12 h, 24 h and 48 h, respectively. The functional analysis of these candidate genes is underway. These candidate genes have great potential in use as multi-biomarkers for organic pollutants and provide a clue in mechanistic aspects of PFOS toxicity in aquatic animals.