Acute effects of heavy metals on the expression of glutathione-related antioxidant genes in the marine ciliate Euplotes crassus SCIE SCOPUS

DC Field Value Language
dc.contributor.author Kim, Se-Hun -
dc.contributor.author Kim, Se-Joo -
dc.contributor.author Lee, Jae-Seong -
dc.contributor.author Lee, Young-Mi -
dc.date.accessioned 2020-04-20T04:40:10Z -
dc.date.available 2020-04-20T04:40:10Z -
dc.date.created 2020-01-28 -
dc.date.issued 2014-08-30 -
dc.identifier.issn 0025-326X -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/2750 -
dc.description.abstract Euplotes crassus, a single-celled eukaryote, is directly affected by environmental contaminants. Here, exponentially cultured E. crassus were exposed to cadmium, copper, lead, and zinc and then the reactive oxygen species (ROS) and total glutathione (GSH) levels were measured. Subsequently, the transcriptional modulation of glutathione peroxidase (GPx) and glutathione reductase (GR) were estimated by quantitative RT-PCR. After an 8-h exposure, significantly higher increases in the relative ROS and total GSH levels were observed in exposed group, compared to the controls. Real-time PCR data revealed that the expression levels of GPx and GR mRNA were sensitively modulated within 8 h of exposure to all heavy metals. These findings suggest that these genes may be involved in cellular defense mechanisms by modulating their gene expression against heavy metal-induced oxidative stress. Thus, they may be useful as potential molecular biomarkers to assess sediment environments for contaminants. (C) 2014 Elsevier Ltd. All rights reserved. -
dc.description.uri 1 -
dc.language English -
dc.publisher PERGAMON-ELSEVIER SCIENCE LTD -
dc.subject INDUCED OXIDATIVE STRESS -
dc.subject ACTIVATED-SLUDGE PLANTS -
dc.subject ACUTE TOXICITY -
dc.subject CADMIUM EXPOSURE -
dc.subject CELLULAR-RESPONSES -
dc.subject END-POINTS -
dc.subject COPPER -
dc.subject TETRAHYMENA -
dc.subject ZINC -
dc.subject ACCUMULATION -
dc.title Acute effects of heavy metals on the expression of glutathione-related antioxidant genes in the marine ciliate Euplotes crassus -
dc.type Article -
dc.citation.endPage 462 -
dc.citation.startPage 455 -
dc.citation.title MARINE POLLUTION BULLETIN -
dc.citation.volume 85 -
dc.citation.number 2 -
dc.contributor.alternativeName 김세주 -
dc.identifier.bibliographicCitation MARINE POLLUTION BULLETIN, v.85, no.2, pp.455 - 462 -
dc.identifier.doi 10.1016/j.marpolbul.2014.05.025 -
dc.identifier.scopusid 2-s2.0-84906264430 -
dc.identifier.wosid 000341556100020 -
dc.type.docType Article; Proceedings Paper -
dc.description.journalClass 1 -
dc.subject.keywordPlus INDUCED OXIDATIVE STRESS -
dc.subject.keywordPlus ACTIVATED-SLUDGE PLANTS -
dc.subject.keywordPlus ACUTE TOXICITY -
dc.subject.keywordPlus CADMIUM EXPOSURE -
dc.subject.keywordPlus CELLULAR-RESPONSES -
dc.subject.keywordPlus END-POINTS -
dc.subject.keywordPlus COPPER -
dc.subject.keywordPlus TETRAHYMENA -
dc.subject.keywordPlus ZINC -
dc.subject.keywordPlus ACCUMULATION -
dc.subject.keywordAuthor Marine ciliate -
dc.subject.keywordAuthor Euplotes crassus -
dc.subject.keywordAuthor Heavy metals -
dc.subject.keywordAuthor Reactive oxygen species -
dc.subject.keywordAuthor Glutathione -
dc.relation.journalWebOfScienceCategory Environmental Sciences -
dc.relation.journalWebOfScienceCategory Marine & Freshwater Biology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Environmental Sciences & Ecology -
dc.relation.journalResearchArea Marine & Freshwater Biology -
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