Acute effects of heavy metals on the expression of glutathione-related antioxidant genes in the marine ciliate Euplotes crassus SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Kim, Se-Hun | - |
dc.contributor.author | Kim, Se-Joo | - |
dc.contributor.author | Lee, Jae-Seong | - |
dc.contributor.author | Lee, Young-Mi | - |
dc.date.accessioned | 2020-04-20T04:40:10Z | - |
dc.date.available | 2020-04-20T04:40:10Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2014-08-30 | - |
dc.identifier.issn | 0025-326X | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2750 | - |
dc.description.abstract | Euplotes crassus, a single-celled eukaryote, is directly affected by environmental contaminants. Here, exponentially cultured E. crassus were exposed to cadmium, copper, lead, and zinc and then the reactive oxygen species (ROS) and total glutathione (GSH) levels were measured. Subsequently, the transcriptional modulation of glutathione peroxidase (GPx) and glutathione reductase (GR) were estimated by quantitative RT-PCR. After an 8-h exposure, significantly higher increases in the relative ROS and total GSH levels were observed in exposed group, compared to the controls. Real-time PCR data revealed that the expression levels of GPx and GR mRNA were sensitively modulated within 8 h of exposure to all heavy metals. These findings suggest that these genes may be involved in cellular defense mechanisms by modulating their gene expression against heavy metal-induced oxidative stress. Thus, they may be useful as potential molecular biomarkers to assess sediment environments for contaminants. (C) 2014 Elsevier Ltd. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject | INDUCED OXIDATIVE STRESS | - |
dc.subject | ACTIVATED-SLUDGE PLANTS | - |
dc.subject | ACUTE TOXICITY | - |
dc.subject | CADMIUM EXPOSURE | - |
dc.subject | CELLULAR-RESPONSES | - |
dc.subject | END-POINTS | - |
dc.subject | COPPER | - |
dc.subject | TETRAHYMENA | - |
dc.subject | ZINC | - |
dc.subject | ACCUMULATION | - |
dc.title | Acute effects of heavy metals on the expression of glutathione-related antioxidant genes in the marine ciliate Euplotes crassus | - |
dc.type | Article | - |
dc.citation.endPage | 462 | - |
dc.citation.startPage | 455 | - |
dc.citation.title | MARINE POLLUTION BULLETIN | - |
dc.citation.volume | 85 | - |
dc.citation.number | 2 | - |
dc.contributor.alternativeName | 김세주 | - |
dc.identifier.bibliographicCitation | MARINE POLLUTION BULLETIN, v.85, no.2, pp.455 - 462 | - |
dc.identifier.doi | 10.1016/j.marpolbul.2014.05.025 | - |
dc.identifier.scopusid | 2-s2.0-84906264430 | - |
dc.identifier.wosid | 000341556100020 | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | INDUCED OXIDATIVE STRESS | - |
dc.subject.keywordPlus | ACTIVATED-SLUDGE PLANTS | - |
dc.subject.keywordPlus | ACUTE TOXICITY | - |
dc.subject.keywordPlus | CADMIUM EXPOSURE | - |
dc.subject.keywordPlus | CELLULAR-RESPONSES | - |
dc.subject.keywordPlus | END-POINTS | - |
dc.subject.keywordPlus | COPPER | - |
dc.subject.keywordPlus | TETRAHYMENA | - |
dc.subject.keywordPlus | ZINC | - |
dc.subject.keywordPlus | ACCUMULATION | - |
dc.subject.keywordAuthor | Marine ciliate | - |
dc.subject.keywordAuthor | Euplotes crassus | - |
dc.subject.keywordAuthor | Heavy metals | - |
dc.subject.keywordAuthor | Reactive oxygen species | - |
dc.subject.keywordAuthor | Glutathione | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.relation.journalWebOfScienceCategory | Marine & Freshwater Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalResearchArea | Marine & Freshwater Biology | - |