Acute effects of heavy metals on the expression of glutathione-related antioxidant genes in the marine ciliate Euplotes crassus SCIE SCOPUS

Cited 16 time in WEB OF SCIENCE Cited 18 time in Scopus
Title
Acute effects of heavy metals on the expression of glutathione-related antioxidant genes in the marine ciliate Euplotes crassus
Author(s)
Kim, Se-Hun; Kim, Se-Joo; Lee, Jae-Seong; Lee, Young-Mi
Publication Year
2014-08-30
Abstract
Euplotes crassus, a single-celled eukaryote, is directly affected by environmental contaminants. Here, exponentially cultured E. crassus were exposed to cadmium, copper, lead, and zinc and then the reactive oxygen species (ROS) and total glutathione (GSH) levels were measured. Subsequently, the transcriptional modulation of glutathione peroxidase (GPx) and glutathione reductase (GR) were estimated by quantitative RT-PCR. After an 8-h exposure, significantly higher increases in the relative ROS and total GSH levels were observed in exposed group, compared to the controls. Real-time PCR data revealed that the expression levels of GPx and GR mRNA were sensitively modulated within 8 h of exposure to all heavy metals. These findings suggest that these genes may be involved in cellular defense mechanisms by modulating their gene expression against heavy metal-induced oxidative stress. Thus, they may be useful as potential molecular biomarkers to assess sediment environments for contaminants. (C) 2014 Elsevier Ltd. All rights reserved.
ISSN
0025-326X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2750
DOI
10.1016/j.marpolbul.2014.05.025
Bibliographic Citation
MARINE POLLUTION BULLETIN, v.85, no.2, pp.455 - 462, 2014
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Subject
INDUCED OXIDATIVE STRESS; ACTIVATED-SLUDGE PLANTS; ACUTE TOXICITY; CADMIUM EXPOSURE; CELLULAR-RESPONSES; END-POINTS; COPPER; TETRAHYMENA; ZINC; ACCUMULATION
Keywords
Marine ciliate; Euplotes crassus; Heavy metals; Reactive oxygen species; Glutathione
Type
Article
Language
English
Document Type
Article; Proceedings Paper
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
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