Development of barcoding technology for verification of microalgae collected from southern coastal region in Korea

Abstract

Countless species occur in the marine microalgal domain. Some are used as health functional foods or medical products but many species are harmful such as those that cause the red tide. Therefore, it is necessary to conduct prompt and accurate identification of microalgal species. As it is quite difficult to accurately distinguish all species in terms of morphology, we performed DNA barcoding analysis using molecular markers for more accurate and rapid screening. DNA chip technology, PCR method, and FISH (fluorescence in situ hybridization) method is a powerful method for studies on microalgal taxonomy and biodiversity. We used the mitochondrial cytochrome c oxidase subunit I (mtCOI) as a barcoding gene to identify microalgal species. In this study, DNA chip assay and PCR method accurately isolated 25 microalgal species and 14 microalgal species, respectively. Also, FISH method showed excellent method in terms of the development of automatic equipment. As a result, we performed DNA chip assay, PCR method, and FISH method for each of microalgal species and determined that the COI barcode gene was suitable as a marker gene, as it could identify various microalgae from the Korean South Sea by species.ccurate identification of microalgal species. As it is quite difficult to accurately distinguish all species in terms of morphology, we performed DNA barcoding analysis using molecular markers for more accurate and rapid screening. DNA chip technology, PCR method, and FISH (fluorescence in situ hybridization) method is a powerful method for studies on microalgal taxonomy and biodiversity. We used the mitochondrial cytochrome c oxidase subunit I (mtCOI) as a barcoding gene to identify microalgal species. In this study, DNA chip assay and PCR method accurately isolated 25 microalgal species and 14 microalgal species, respectively. Also, FISH method showed excellent method in terms of the development of automatic equipment. As a result,