Three novel superoxide dismutase genes identified in the marine polychaete Perinereis nuntia and their differential responses to single and combined metal exposures SCIE SCOPUS

Cited 11 time in WEB OF SCIENCE Cited 13 time in Scopus
Title
Three novel superoxide dismutase genes identified in the marine polychaete Perinereis nuntia and their differential responses to single and combined metal exposures
Author(s)
Won, Eun-Ji; Ra, Kongtae; Kim, Kyung-Tae; Lee, Jae-Seong; Lee, Young-Mi
KIOST Author(s)
Ra, Kong Tae(나공태)Kim, Kyung Tae(김경태)
Publication Year
2014-09
Abstract
To identify superoxide dismutase (SOD) genes and evaluate their usefulness as potential markers for monitoring metal toxicity in aquatic environment, we cloned, sequenced, and characterized 3 SOD genes (Cu/Zn-SOD1, Cu/Zn-SOD2, and Mn-SOD) from the marine polychaete Perinereis nuntia. The accumulated metal contents and expressions of 3 SOD genes were compared after exposure to single and combinations of heavy metals, As, Ni, and Pb. The deduced amino acid sequences of the 3 SODs had evolutionary conserved domains, such as metal binding sites, and signature sequences. The phylogenetic analysis revealed that Cu/Zn-SOD1, Cu/Zn-SOD2, and Mn-SOD were clustered with extracellular Cu/Zn-SOD, intracellular Cu/Zn-SOD and mitochondrial Mn-SOD, respectively, of other species. The accumulated contents of Ni and Pb increased significantly in a time - dependent manner after exposure to both single and combination of the metals. However, the concentration of As did not change significantly in the exposure test. The quantitative real-time polymerase chain reaction (PCR) array showed that the 3 SOD genes had differential expression patterns depending on the exposure condition. The expression of all SODs mRNAs was significantly elevated in response to Pb alone and in combination with As. The mRNA level of Cu/Zn-SOD1 was the highest after exposure to Pb alone, while that of Mn-SOD was remarkably enhanced after exposure to a combination of As and Pb. Exposure to Ni alone rapidly elevated the expression of Cu/Zn-SOD1 and Mn-SOD mRNA, which then gradually decreased. Exposure to As had no significant effect on the modulation of any of the SOD genes of P. nuntia. These results suggest that all SOD genes might play important roles in cellular protection as antioxidant enzymes against heavy metal toxicity via different modes of action in P. nuntia and might have the potential to act as indicators in an environment containing a mixture of metals. (C) 2014 Elsevier Inc. All rights reserved.
ISSN
0147-6513
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2732
DOI
10.1016/j.ecoenv.2014.03.026
Bibliographic Citation
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v.107, pp.36 - 45, 2014
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Subject
MESSENGER-RNA EXPRESSION; OXIDATIVE STRESS; MOLECULAR-CLONING; NEREIS-DIVERSICOLOR; ANTIOXIDANT ENZYMES; TROPHIC TRANSFER; TRACE-METALS; IN-VITRO; COPPER; SEDIMENTS
Keywords
Superoxide dismutase (SOD); Polychaete Perinereis nuntia; Heavy metal; Biomarker
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Related Researcher
Research Interests

Management of marine environment,Marine pollution,Trace metal(Heavy metal),해양환경관리,해양오염,미량금속(중금속)

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