H2 Production from Steel Mill Waste Gas using a Carboxydotrophic Hydrogenogen

DC Field Value Language
dc.contributor.author 이현숙 -
dc.contributor.author 김민식 -
dc.contributor.author 김윤재 -
dc.contributor.author 배승섭 -
dc.contributor.author 김태완 -
dc.contributor.author 이정현 -
dc.contributor.author 강성균 -
dc.date.accessioned 2020-07-16T09:32:24Z -
dc.date.available 2020-07-16T09:32:24Z -
dc.date.created 2020-02-11 -
dc.date.issued 2013-05-01 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/27103 -
dc.description.abstract Hydrogenogenic CO oxidation (CO + H2O → CO2 + H2) has the potential for H2 production as a clean renewable fuel. Thermococcus onnurineus NA1, which grows on CO and produces H2, has a unique gene cluster encoding the carbon monoxide dehydrogenase and the hydrogenase. MC01 mutant, with the gene cluster being placed under the control of a strong promoter, showed 30-fold higher transcription of the mRNA encoding CODH, hydrogenase, and Na+/H+ antiporter, and a 1.8-fold higher specific activity for CO-dependent H2 production compared to the wild type strain. The H2 production potential of the MC01 mutant in a bioreactor culture was 3.8-fold higher than that of the wild type strain. The engineered strain possessed high activity for the bioconversion of industrial waste gases created as a by-product during steel production. This work represents the first demonstration of H2 production from steel mill waste gas using a carboxydotrophic hydrogenogenic microbe.ydrogenase and the hydrogenase. MC01 mutant, with the gene cluster being placed under the control of a strong promoter, showed 30-fold higher transcription of the mRNA encoding CODH, hydrogenase, and Na+/H+ antiporter, and a 1.8-fold higher specific activity for CO-dependent H2 production compared to the wild type strain. The H2 production potential of the MC01 mutant in a bioreactor culture was 3.8-fold higher than that of the wild type strain. The engineered strain possessed high activity for the bioconversion of industrial waste gases created as a by-product during steel production. This work represents the first demonstration of H2 production from steel mill waste gas using a carboxydotrophic hydrogenogenic microbe. -
dc.description.uri 2 -
dc.language English -
dc.publisher 한국미생물학회 -
dc.relation.isPartOf International Meeting of the Microbiological Society of Korea -
dc.title H2 Production from Steel Mill Waste Gas using a Carboxydotrophic Hydrogenogen -
dc.type Conference -
dc.citation.conferencePlace KO -
dc.citation.endPage 284 -
dc.citation.startPage 284 -
dc.citation.title International Meeting of the Microbiological Society of Korea -
dc.contributor.alternativeName 이현숙 -
dc.contributor.alternativeName 김민식 -
dc.contributor.alternativeName 김윤재 -
dc.contributor.alternativeName 배승섭 -
dc.contributor.alternativeName 김태완 -
dc.contributor.alternativeName 이정현 -
dc.contributor.alternativeName 강성균 -
dc.identifier.bibliographicCitation International Meeting of the Microbiological Society of Korea, pp.284 -
dc.description.journalClass 2 -
Appears in Collections:
Marine Resources & Environment Research Division > Marine Biotechnology &Bioresource Research Department > 2. Conference Papers
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