H2 Production from Steel Mill Waste Gas using a Carboxydotrophic Hydrogenogen

Title
H2 Production from Steel Mill Waste Gas using a Carboxydotrophic Hydrogenogen
Author(s)
이현숙; 김민식; 김윤재; 배승섭; 김태완; 이정현; 강성균
KIOST Author(s)
Lee, Hyun Sook(이현숙)Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)
Publication Year
2013-05-01
Abstract
Hydrogenogenic CO oxidation (CO + H2O → CO2 + H2) has the potential for H2 production as a clean renewable fuel. Thermococcus onnurineus NA1, which grows on CO and produces H2, has a unique gene cluster encoding the carbon monoxide dehydrogenase and the hydrogenase. MC01 mutant, with the gene cluster being placed under the control of a strong promoter, showed 30-fold higher transcription of the mRNA encoding CODH, hydrogenase, and Na+/H+ antiporter, and a 1.8-fold higher specific activity for CO-dependent H2 production compared to the wild type strain. The H2 production potential of the MC01 mutant in a bioreactor culture was 3.8-fold higher than that of the wild type strain. The engineered strain possessed high activity for the bioconversion of industrial waste gases created as a by-product during steel production. This work represents the first demonstration of H2 production from steel mill waste gas using a carboxydotrophic hydrogenogenic microbe.ydrogenase and the hydrogenase. MC01 mutant, with the gene cluster being placed under the control of a strong promoter, showed 30-fold higher transcription of the mRNA encoding CODH, hydrogenase, and Na+/H+ antiporter, and a 1.8-fold higher specific activity for CO-dependent H2 production compared to the wild type strain. The H2 production potential of the MC01 mutant in a bioreactor culture was 3.8-fold higher than that of the wild type strain. The engineered strain possessed high activity for the bioconversion of industrial waste gases created as a by-product during steel production. This work represents the first demonstration of H2 production from steel mill waste gas using a carboxydotrophic hydrogenogenic microbe.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/27103
Bibliographic Citation
International Meeting of the Microbiological Society of Korea, pp.284, 2013
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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