Anti-Inflammation Activities of Mycosporine-Like Amino Acids (MAAs) in Response to UV Radiation Suggest Potential Anti-Skin Aging Activity SCIE SCOPUS

Cited 62 time in WEB OF SCIENCE Cited 69 time in Scopus
Title
Anti-Inflammation Activities of Mycosporine-Like Amino Acids (MAAs) in Response to UV Radiation Suggest Potential Anti-Skin Aging Activity
Author(s)
Suh, Sung-Suk; Hwang, Jinik; Park, Mirye; Seo, Hyo Hyun; Kim, Hyoung-Shik; Lee, Jeong Hun; Moh, Sang Hyun; Lee, Taek-Kyun
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2014-10
Abstract
Certain photosynthetic marine organisms have evolved mechanisms to counteract UV-radiation by synthesizing UV-absorbing compounds, such as mycosporine-like amino acids (MAAs). In this study, MAAs were separated from the extracts of marine green alga Chlamydomonas hedleyi using HPLC and were identified as porphyra-334, shinorine, and mycosporine-glycine (mycosporine-Gly), based on their retention times and maximum absorption wavelengths. Furthermore, their structures were confirmed by triple quadrupole MS/MS. Their roles as UV-absorbing compounds were investigated in the human fibroblast cell line HaCaT by analyzing the expression levels of genes associated with antioxidant activity, inflammation, and skin aging in response to UV irradiation. The mycosporine-Gly extract, but not the other MAAs, had strong antioxidant activity in the 2,2-diphenyl-1-picryhydrazyl (DPPH) assay. Furthermore, treatment with mycosporine-Gly resulted in a significant decrease in COX-2 mRNA levels, which are typically increased in response to inflammation in the skin, in a concentration-dependent manner. Additionally, in the presence of MAAs, the UV-suppressed genes, procollagen C proteinase enhancer (PCOLCE) and elastin, which are related to skin aging, had increased expression levels equal to those in UV-mock treated cells. Interestingly, the increased expression of involucrin after UV exposure was suppressed by treatment with the MAAs mycosporine-Gly and shinorine, but not porphyra-334. This is the first report investigating the biological activities of microalgae-derived MAAs in human cells.
ISSN
1660-3397
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2709
DOI
10.3390/md12105174
Bibliographic Citation
MARINE DRUGS, v.12, no.10, pp.5174 - 5187, 2014
Publisher
MDPI AG
Subject
SOLAR ULTRAVIOLET-RADIATION; IN-VIVO; ANTIOXIDANT ACTIVITY; B RADIATION; PORPHYRA-334; CYANOBACTERIA; PHOTOSYNTHESIS; PHYTOPLANKTON; BIOSYNTHESIS; MICROALGAE
Keywords
mycosporine-like amino acids (MAAs); HPLC; mycosporine-glycine; shinorine; porphyra-334
Type
Article
Language
English
Document Type
Article
Publisher
MDPI AG
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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