Molecular cloning, characterization and expression analysis of lipopolysaccharide-induced TNF-a factor (LITAF) gene from rock bream

Title
Molecular cloning, characterization and expression analysis of lipopolysaccharide-induced TNF-a factor (LITAF) gene from rock bream
Author(s)
채영선; 김하나; 김주원; 황성돈; 정지현; 심원준; 박찬일
KIOST Author(s)
Jung, Jee Hyun(정지현)Shim, Won Joon(심원준)
Publication Year
2013-05-08
Abstract
The lipopolysaccharide-induced TNF-a factor (LITAF) is an important cytokine toregulate the innate immunity which plays major roles in regulating inflammatory, apoptosisand immune related genes. In this study, we identified and characterized rock bream(Oplegnathusfasciatus) LITAF gene. The full-length cDNA of LITAF1 and LITAF2 consistof 163 and 138 amino acids with a conserved LITAF domain that contain Zinc (Zn2+)binding structure and two of CXXC motif by multiple alignment. The expression analysisof LITAF1 mRNA in various tissue were performed using Quantitative real-time PCR. Thehighest mRNA expression level was detected in intestine. On the other hand, mRNAexpression of LITAF2 was detected in gill. In the expression analysis, mRNA expressionlevel of LITAF1 was significantly induced when infected with E. tarda, whereas, theexpression level of LITAF2 was induced by infection of S. iniae. LITAFs could beinduced by LPS (lipopolysaccharide), Gram-negative bacterial outer membrane. LITAF2was induced by Gram-positive bacterial, S. iniae. LITAF1 and 2 of rock bream hasimportant roles in infection such as activation of TNFSF (tumor necrosis factorsuperfamily) promoter.rized rock bream(Oplegnathusfasciatus) LITAF gene. The full-length cDNA of LITAF1 and LITAF2 consistof 163 and 138 amino acids with a conserved LITAF domain that contain Zinc (Zn2+)binding structure and two of CXXC motif by multiple alignment. The expression analysisof LITAF1 mRNA in various tissue were performed using Quantitative real-time PCR. Thehighest mRNA expression level was detected in intestine. On the other hand, mRNAexpression of LITAF2 was detected in gill. In the expression analysis, mRNA expressionlevel of LITAF1 was significantly induced when infected with E. tarda, whereas, theexpression level of LITAF2 was induced by infection of S. iniae. LITAFs could beinduced by LPS (lipopolysaccharide), Gram-negative bacterial outer membrane. LITAF2was induced by Gram-positive bacterial, S. iniae. LITAF1 and 2 of rock bream hasimportant roles in infection such as activation of TNFSF (tumor necrosis factorsuperfamily) promoter.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/27085
Bibliographic Citation
한국해양학회, pp.2494, 2013
Publisher
한국해양학회
Type
Conference
Language
English
Publisher
한국해양학회
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

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