Molecular cloning, characterization and expression analysis of lipopolysaccharide-induced TNF-a factor (LITAF) gene from rock bream

Abstract

The lipopolysaccharide-induced TNF-a factor (LITAF) is an important cytokine toregulate the innate immunity which plays major roles in regulating inflammatory, apoptosisand immune related genes. In this study, we identified and characterized rock bream(Oplegnathusfasciatus) LITAF gene. The full-length cDNA of LITAF1 and LITAF2 consistof 163 and 138 amino acids with a conserved LITAF domain that contain Zinc (Zn2+)binding structure and two of CXXC motif by multiple alignment. The expression analysisof LITAF1 mRNA in various tissue were performed using Quantitative real-time PCR. Thehighest mRNA expression level was detected in intestine. On the other hand, mRNAexpression of LITAF2 was detected in gill. In the expression analysis, mRNA expressionlevel of LITAF1 was significantly induced when infected with E. tarda, whereas, theexpression level of LITAF2 was induced by infection of S. iniae. LITAFs could beinduced by LPS (lipopolysaccharide), Gram-negative bacterial outer membrane. LITAF2was induced by Gram-positive bacterial, S. iniae. LITAF1 and 2 of rock bream hasimportant roles in infection such as activation of TNFSF (tumor necrosis factorsuperfamily) promoter.rized rock bream(Oplegnathusfasciatus) LITAF gene. The full-length cDNA of LITAF1 and LITAF2 consistof 163 and 138 amino acids with a conserved LITAF domain that contain Zinc (Zn2+)binding structure and two of CXXC motif by multiple alignment. The expression analysisof LITAF1 mRNA in various tissue were performed using Quantitative real-time PCR. Thehighest mRNA expression level was detected in intestine. On the other hand, mRNAexpression of LITAF2 was detected in gill. In the expression analysis, mRNA expressionlevel of LITAF1 was significantly induced when infected with E. tarda, whereas, theexpression level of LITAF2 was induced by infection of S. iniae. LITAFs could beinduced by LPS (lipopolysaccharide), Gram-negative bacterial outer membrane. LITAF2was induced by Gram-positive bacterial, S. iniae. LITAF1 and 2 of rock bream hasimportant roles in infection such as activation of TNFSF (tumor necrosis factorsuperfamily) promoter.