A Study on the Acute Toxicity of Iranian Heavy

Title
A Study on the Acute Toxicity of Iranian Heavy
Author(s)
김하나; 채영선; 정지현; 심원준
KIOST Author(s)
Jung, Jee Hyun(정지현)Shim, Won Joon(심원준)
Publication Year
2013-05-25
Abstract
This study examines whether acute toxic responses played by the innate immune systemdepend on which metabolic pathway was induced after exposure to crude oil. We exposedjuvenile rockfish to spilled crude oil (Iranian Heavy Crude Oil IHCO in gelatin capsules)from the “Hebei spirit” oil spill by feeding. The effects on multiple fish hepatodetoxificationenzymes (Cytochrome P4501A and GST) and the expression level of the immune responsegenes, including interleukin-1beta (IL-1β), granulocyte colony-stimulating factor (G-CSF)and Cathepsin L (CTSL), were measured in the liver, spleen and kidney. The oil-fed fishhad significantly higher concentrations of biliary fluorescent metabolites and CYP1Aexpression during the initial stage after exposure (12~48 h after exposure) than those inthe liver and kidney of the control group. Similarly, the highest mRNA expression levels ofIL-1β and G-CSF were detected in the liver at the early stages of exposure (12 h afterexposure). Following exposure, the levels of ISG15 and G-CSF mRNA remained high at120 h after treatment in the liver but the levels of IL-1β and CTSL gradually decreased toan expression level equal to or less than the control group. Our data suggest that theinnate immune and hepatodetoxification responses in oil-fed fish were induced at the initialstage of exposure to the IHCO at the same time but several immune-related genesdecreased tCO in gelatin capsules)from the “Hebei spirit” oil spill by feeding. The effects on multiple fish hepatodetoxificationenzymes (Cytochrome P4501A and GST) and the expression level of the immune responsegenes, including interleukin-1beta (IL-1β), granulocyte colony-stimulating factor (G-CSF)and Cathepsin L (CTSL), were measured in the liver, spleen and kidney. The oil-fed fishhad significantly higher concentrations of biliary fluorescent metabolites and CYP1Aexpression during the initial stage after exposure (12~48 h after exposure) than those inthe liver and kidney of the control group. Similarly, the highest mRNA expression levels ofIL-1β and G-CSF were detected in the liver at the early stages of exposure (12 h afterexposure). Following exposure, the levels of ISG15 and G-CSF mRNA remained high at120 h after treatment in the liver but the levels of IL-1β and CTSL gradually decreased toan expression level equal to or less than the control group. Our data suggest that theinnate immune and hepatodetoxification responses in oil-fed fish were induced at the initialstage of exposure to the IHCO at the same time but several immune-related genesdecreased t
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26969
Bibliographic Citation
한국해양학술회, pp.2497, 2013
Publisher
한국해양학회
Type
Conference
Language
English
Publisher
한국해양학회
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse