A Novel filamentous Cyanobacterium Leptolyngbya sp. KIOST-1 as a potential candidate for bio-resource production

Abstract

In this study, we report a filamentous cyanobacterium that was isolated from the microalgae culture pond in Korea. Morphological analysis revealed that the non-motile filaments were composed of single trichomes that were typically consisting of cylindrical cells, and akinetes, false-branching, gas vacuoles, heterocysts or sheaths were not found from it. To determine the taxonomical position of the isolate, 16S rRNA and 16S-23S intergenic spacer (ITS) region sequences were obtained and compared with the GenBank database. The results revealed that the strain belonged to the genus Leptolyngbya and finally designated as Leptolyngbya sp. KIOST-1. To ascertain taxonomical position of the isolate, phylogenetic analysis were conducted using 16S rRNA and 16S-23S ITS sequences. The cellular components in the isolate were further investigated, and carbohydrate and lipid contents were estimated to be 24.50 ± 0.70% and 11.35 ± 0.47%, respectively. Additionally, its protein content was estimated to be 52.64 ± 0.12%, thus indicating that it is the major component in the lyophilized Leptolyngbya sp. KIOST-1 cells. Based on these results, the newly isolated Leptolyngbya sp. KIOST-1 might be considered as a potential candidate for bio-resource production, and further studies are currently in process to investigate the genome of the isolate.g of cylindrical cells, and akinetes, false-branching, gas vacuoles, heterocysts or sheaths were not found from it. To determine the taxonomical position of the isolate, 16S rRNA and 16S-23S intergenic spacer (ITS) region sequences were obtained and compared with the GenBank database. The results revealed that the strain belonged to the genus Leptolyngbya and finally designated as Leptolyngbya sp. KIOST-1. To ascertain taxonomical position of the isolate, phylogenetic analysis were conducted using 16S rRNA and 16S-23S ITS sequences. The cellular components in the isolate were further investigated, and carbohydrate and lipid contents were estimated to be 24.50 ± 0.70% and 11.35 ± 0.47%, respectively. Additionally, its protein content was estimated to be 52.64 ± 0.12%, thus indicating that it is the major component in the lyophilized Leptolyngbya sp. KIOST-1 cells. Based on these results, the newly isolated Leptolyngbya sp. KIOST-1 might be considered as a potential candidate for bio-resource production, and further studies are currently in process to investigate the genome of the isolate.